In order to preserve the wild resources and meet the needs of cultivation, with the method of tissue culture, tender stems of Draba nemorosa were used as material to study on callus inducement and differentiation, rooting of adventitious buds, rooting multiplication of tube seedlings, replantation and field planting. The results showed that MS+6-BA 0.4 mg/L+2,4-D 2.5 mg/L was the ideal medium for inducing callus and callus multiplication; MS+6-BA 0.6 mg/L+NAA 0.1 mg/L was suitable for callus differentiation and multiplication of adventitious buds; the best medium for rooting of adventitious buds and rooting multiplication was 1/3MS+0.6 mg/L; the transplanting survival rate of tube seedlings was 86.8% and stable planting survival rate was 96.4%; the seedlings planted in field were maintained all traits of the wild Draba nemorosa.
ZHU Ting-ting
,
YAN Wei-yu
,
LI Xiang-fen
,
NING Shu-xiang
,
JIANG Chang-yang
. The Study on Callus Inducement and Differentiation of Draba nemorosa[J]. Subtropical Plant Science, 2012
, 41(02)
: 23
-26
.
DOI: 10.3969/j.issn.1009-7791.2012.02.006
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