To select the optimum purifying process of total flavonoids in Pyrrosia petiolosa, orthogonal experiment was designed with the desorption rate of total flavonoids and the content of total flavonoids as indexes. The results showed that the best separation conditions were determined as: sample concentration 0.5 mg.mL-1 at pH 6, eluent concentration 50%, elution rate 2 mL.min-1 by D-101 macroporous resin. The concentration of total flavonoids in the filtrate could reach 76.40%, and the method was stable and feasible, and the result was reliable.
LIU Jing
,
CHENG Dan-dan
,
XIN Xiao-wei
,
GAO De-min
,
ZHANG Hong-meng
. Optimization of Separation and Purification Procedure of Total Flavonoids in Pyrrosia petiolosa by Macroporous Adsorption Resin[J]. Subtropical Plant Science, 2014
, 43(03)
: 202
-205
.
DOI: 10.3969/j.issn.1009-7791.2014.03.004
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