Subtropical Plant Science >

2025 , Vol. 54 >Issue 6: 653 - 659

DOI: https://doi.org/10.3969/j.issn.1009-7791.2025.06.007

Research articles

Impacts of Temperature Stress on Photosynthesis Characteristics of Paphiopedilum Species

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  • (1. College of Landscape and Art, Fujian Agriculture and Forestry University, Fuzhou 350108, Fujian China; 2. Eastern China Conservation Centre for Wild Endangered Plant Resources, Shanghai Chenshan Botanical Garden, Shanghai 201602, China; 3. Department of Botany, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510405, Guangdong China)

Received date: 2025-03-06

  Accepted date: 2025-04-09

  Online published: 2025-12-31

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Abstract

Paphiopedilum glaucophyllum, P. charlesworthii, and P. malipoense, which differ in habitat and altitude, were selected to investigate the effects of different temperature stress (low, normal, and high) on the photosynthetic characteristics of Paphiopedilum. Three temperature gradients were set: low-temperature stress (15 ℃), room temperature (22 ℃, CK), and high-temperature stress (30 ℃), with each gradient subjected to short-term (1 d) and long-term (7 d) treatments. Leaf gas exchange parameters (net photosynthetic rate Pn, stomatal conductance Gs, transpiration rate Tr) and chlorophyll fluorescence parameters were measured. The results showed that, in terms of gas exchange parameters, Pn, Gs, and Tr of the three species all increased under long-term high-temperature stress. Conversely, Pn, Gs, and Tr were decreased under long-term low-temperature stress, except for P. malipoense. In terms of chlorophyll fluorescence parameters, the initial fluorescence (Fo) was generally increased under long-term temperature stresses. The maximum fluorescence (Fm), the potential photochemical efficiency of photosystem Ⅱ (PSⅡ) (Fv/Fo), and the maximum photochemical efficiency of PSⅡ (Fv/Fm) of three species all exhibited an increase under high-temperature stress but decreased under low-temperature stress. These findings demonstrated that the photosynthesis in three Paphiopedilum species was inhibited by both low and high temperature stresses, with greater pronounced suppression at low temperatures. These results indicated that P. malipoense possesses a stronger adaptive capacity under low and high temperature stresses, while P. charlesworthii and P. glaucophyllum adapted better to high temperatures. This study provides insights into the physiological response mechanisms of three Paphiopedilum species from different habitats to low and high temperature stresses, offering theoretical support for the introduction, conservation, and cultivation of Paphiopedilum species in similar habitats.

Key words： Paphiopedilum; temperature stress; photoinhibition; environmental adaptation

Cite this article

PAN Ren-fu, HU Chao, GONG Hai-guang, LI Ming-he, HUANG Wei-chang . Impacts of Temperature Stress on Photosynthesis Characteristics of Paphiopedilum Species[J]. Subtropical Plant Science, 2025 , 54(6) : 653 -659 . DOI: 10.3969/j.issn.1009-7791.2025.06.007

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