Subtropical Plant Science >

2026 , Vol. 55 >Issue 1: 64 - 72

DOI: https://doi.org/10.3969/j.issn.1009-7791.2026.01.007

Research articles

Isolation and Identification of Major Pathogenic Fungi During Castanea henryi Storage

Expand
  • (1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 2. College of Tea and Food Sciences, Wuyi University, Wuyishan 354300, Fujian China; 3. Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Province University, Fuzhou 350002, Fujian China)

Received date: 2025-10-15

  Accepted date: 2026-01-06

  Online published: 2026-02-28

Fold

Abstract

Castanea henryi is susceptible to spoilage during storage, primarily due to pathogenic infection. In this study, the main cultivated variety C. henryi 'Youzhen' in northern Fujian were used as experimental material. Pathogenic fungi were isolated and purified from decayed kernel tissues, followed by identification based on morphological and molecular biological methods. A phylogenetic tree was constructed to clarify the taxonomic status of the isolated strains. The results showed that the pathogens responsible for C. henryi spoilage included Gd21Ⅱ, Dd13, Dc21, Ga11, Gd21Ⅰ, Bc21, Gd12Ⅱ, Fd12Ⅱ, Ad11, Fd12Ⅰ，Fd13, Dd11, and Gb23, which were respectively classified as Penicillium mallochii, Cladosporium sp., Botryosphaeria dothidea, Debaryomyces robertsiae, Candida sp., Diaporthe sp., and Pseudomonas oryzihabitans. Through the isolation and identification of major pathogenic fungi during storage, this study clarifies the species and phylogenetic classification of the spoilage-causing microorganisms, providing a theoretical basis for the development of postharvest storage and preservation techniques for C. henryi. It also provides a theoretical basis for the development of new preservation technologies for C. henryi.

Key words： Castanea henryi; isolation and purification; pathogen; identification

Cite this article

LI Hui-yao, LIN Shu-ting, FAN Zi-wei, MA Chun-hua, FU Xin-zheng, CHEN Yi-hui, ZHANG Jing . Isolation and Identification of Major Pathogenic Fungi During Castanea henryi Storage[J]. Subtropical Plant Science, 2026 , 55(1) : 64 -72 . DOI: 10.3969/j.issn.1009-7791.2026.01.007

References

[1]  杨恒, 吴才鑫. 锥栗果用林营建技术[J]. 现代农业科技, 2025(14): 89–92.

[2]  Zhao Y F, Lin H T, Lin Y F, Chen Y H, Ming Y L. Effect of heat treatment on browning delaying and phenolics metabolism in pericarp of harvested longan fruit [J]. Modern Food Science and Technology, 2014, 30(5): 218–224.

[3]  江锡兵, 吴聪连, 姚进, 吴剑, 胡伟云, 吴小云, 苏光浪, 王衍鹏, 赖俊声, 龚榜初. 锥栗新品种‘YLZ 1号’[J]. 园艺学报, 2024, 51(S2): 49–50.

[4]  陈水电. 建瓯市锥栗种植现状及造林管理技术[J]. 现代园艺, 2013(4): 28–29.

[5]  杨春华. 浅谈建瓯锥栗经济问题[J]. 中国林业产业, 2024(3): 49–51.

[6]  Tu X D, Lin W J, Xin Y X, Fy H H, Zhou C Y. Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family [J]. Molecular Horticulture, 2025, 5(1): 33.

[7]  Li J, Xiang N, Chen J, Shu Z C, Guo X B. Vitamin E and carotenoid accumulation during kernel development in two varieties of Castanea henryi [J]. International Journal of Food Science and Technology, 2021, 56(12): 6539–6548.

[8]  叶树涛, 顾光仕, 李煜, 方舟, 张晶, 杨金辉, 郑国华, 陈辉. 锥栗农家品种栗疫病抗性及果实表型性状的变异分析与联合选择[J]. 经济林研究, 2022, 40(1): 82–94.

[9]  林忠. 寿宁县锥栗炭疽病的发生与综合防治[J]. 绿色科技, 2019(17): 118–119.

[10] 董爱荣, 于文旭, 王思涵, 孙宁. 水曲柳枝枯病病病原菌分离鉴定、生物学特性及发病前后真菌多样性分析[J/OL]. 高原农业, 2025, 9(4): 421–431.

[11]  李新茹, 罗义, 李红基, 肖扬. 羊肚菌白霉病和蛛网病的病原菌验证及菌丝体培养特性分析[J]. 食药用菌, 2025, 33(4): 272–279.

[12]  Chang Y T, Liu P Y, Chang F H, Shiah F K, Hsieh C H, Lu H P. Characterization of prokaryotic plankton community structure in the Southern East China Sea using combined 16S-rDNA and 16S-rRNA [J]. Scientific Reports, 2025, 15(1): 29896.

[13]  D’Humieres C, Haviari S, Petitjean N, Deconinck L, Gueye S. Comparison of clinical metagenomics with 16S rDNA Sanger sequencing for the bacteriological diagnosis of culture-negative samples [J]. International Journal of Medical Microbiology, 2025, 318: 151650.

[14]  罗静初. 序列数据库搜索系统BLAST简介[J]. 生物信息学, 2025, 23(3): 165–174.

[15]  李欣, 邢帆, 田淑月, 陈璇羽, 王逢会, 王欣怡, 张静, 陈波波. 海洋青霉属真菌次生代谢产物及生物活性的研究进展[J]. 中国海洋药物, 2025, 44(4): 86–98.

[16]  孙园园, 于豪冰, 翟祥宁, 宁哲, 何颖, 胡波, 刘小宇. 青霉属真菌次级代谢基因挖掘的研究进展[J]. 中国海洋药物, 2024, 43(1): 74–85.

[17]  Prencipe S, Siciliano I, Gatti C, Garibaldi A, Gullino M L, Botta R, Spadaro D.    Several species of Penicillium isolated from chestnut flour processing are pathogenic on fresh chestnuts and produce mycotoxins [J]. Food Microbiology, 2018, 76: 396–404.

[18]  Hu Q, An T, Jia W, Chen J X, Xu Y, Li Z, Ding H X. Botryosphaeria dothidea causes leaf spot disease on Rosa roxburghii in China [J]. Crop Protection, 2025, 197: 107312.

[19]  Tong X Y, Song L L, Du H F, Zhang Y H, Shou J W. High efficacy of (Z)-ligustilide in inhibiting postharvest apple ring rot and its antifungal mechanism against Botryosphaeria dothidea [J]. Postharvest Biology and Technology, 2025, 228: 113671.

[20]  Rathod B G, Poosarla V G, Rajagopalan G, Rajagopalan G, Kumari A. Bioethanol production from fruit waste using a microbial co-culture of Bacillus siamensis F2 and Candida albicans GP1 [J]. Biofuels Bioproducts & Biorefining–Biofpr, 2025, 19(6): 1915.

[21]  Zhao L, Ren X, Wen R. Physiological mechanisms and transcriptomic analysis of the disease resistance in pear fruit induced by Debaryomyces hansenii [J]. International Journal of Food Microbiology, 2025, 442: 111350.

[22]  张娜娜, 温晓蕾, 李双民, 冯丽娜, 霍佳欢, 兰淑慧, 栗佳宁, 郭思柔, 王建华, 齐慧霞. 三种镰刀菌引起的板栗内腐病病原菌鉴定[J]. 中国农业科技导报, 2022, 24(7): 117–122.

[23]  Miller A C, Iver M L L. The disease triangle of chestnut: A review of host, pathogen, and environmental interactions of chestnuts cultivated in the Eastern United States [J]. Plant Disease, 2025, 109(2): 245–256.

[24]  Nie X H, Yan B Q, Liu S, Chu S H, Fang K F, Liu Y, Qin L, Xing Y. Molecular genetic analysis of natural introgression to enhance chestnut blight resistance of Castanea henryi var. omeiensis [J]. Industrial Crops and Products, 2024, 215: 118660.

[25]  李萌, 彭丽桃, 张曦晨, 杨书珍, 王玉. 板栗采后病原菌的分离及其控制研究[C/OL]//植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集. 贵阳: 中国植物病理学会, 2021: 667.

[26]  Li M, Yang S Z, Peng L T, Zeng K F, Fang K F, Feng B R, Yang J J. Compositional shifts in fungal community of chestnuts during storage and their correlation with fruit quality [J]. Postharvest Biology and Technology, 2022, 191: 111983.

[27]  Zeng Q, Wang L, Long S, Dong W L, Li Y Y, Chen Y X, Zhou G. Inhibitory effects and mechanisms of perilla essential oil and perillaldehyde against chestnut pathogen Botryosphaeria dothidea [J]. Journal of Fungi, 2024, 10(8): 526.

Options
Outlines

/

〈 〉

Copyright © Editorial office of Subtropical Plant Science
Tel: 0592-5654157　E-mail: yrdzwkx@126.com
Supported by Beijing Magtech Co., Ltd.