Subtropical Plant Science >

2026 , Vol. 55 >Issue 2: 144 - 152

DOI: https://doi.org/10.3969/j.issn.1009-7791.2026.02.002

Research articles

Molecular Identification of Polygonatum cyrtonema and P. sibiricum in Hubei Region Based on ETS Sequences

Expand
  • (1. School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, Hubei China; 2. School of Public Health, Wuhan University, Wuhan 430071, Hubei China)

Received date: 2025-11-26

  Accepted date: 2026-01-26

  Online published: 2026-04-30

Fold

Abstract

The external transcribed spacer (ETS) molecular marker was used to identify and analyze Polygonatum cyrtonema and P. sibiricum from Hubei Province, to provide a basis for their efficient discrimination. A total of five individuals of P. cyrtonema and eleven of P. sibiricum were collected from Hubei Province for molecular analysis. The ETS sequence was amplified by PCR, followed by sequence feature analysis using bioinformatics methods. A phylogenetic tree was constructed using the neighbor-joining (NJ) method. Results showed that the ETS sequences of both species were 1 000 bp in length, yet their nucleotide compositions differed markedly. In P. cyrtonema, GC content was 41.6%-45.1%, with an average intraspecific genetic distance of 0.208 and an intraspecific sequence similarity of 88.1%, containing 134 parsimony informative sites. In P. sibiricum, GC content was significantly higher by 56.5%-61.5%, the average intraspecific genetic distance was 0.131, and the sequence similarity was 79.2%, with 298 parsimony informative sites. In the NJ phylogenetic tree, P. cyrtonema and P. sibiricum formed clearly separated clades, enabling effective discrimination. The ETS sequence can serve as a species-specific molecular marker for distinguishing P. cyrtonema and P. sibiricum, providing a molecular basis for precise germplasm identification and genetic diversity analysis of the two species, as well as for quality control of Hubei's local Polygonatum medicinal materials.

Key words： Polygonatum cyrtonema; Polygonatum sibiricum; external transcribed spacer (ETS); molecular identification

Cite this article

ZHANG Jun-zhuo, XIONG Chao, XIANG Heng, WANG Yong-cui, XU Ran, MA Zhao-cheng . Molecular Identification of Polygonatum cyrtonema and P. sibiricum in Hubei Region Based on ETS Sequences[J]. Subtropical Plant Science, 2026 , 55(2) : 144 -152 . DOI: 10.3969/j.issn.1009-7791.2026.02.002

References

[1]  中国科学院中国植物志编辑委员会. 中国植物志(第15卷) [M]. 北京: 科学出版社, 1978．

[2]  苏文田, 刘跃钧, 蒋燕锋, 谢建秋, 潘心禾, 刘京晶, 斯金平. 黄精产业发展现状与可持续发展的建议[J]. 中国中药杂志, 2018, 43(13): 2831–2835.

[3]  国家药典委员会. 中华人民共和国药典：2020年版一部[M]. 北京: 中国医药科技出版社, 2020: 319–320.

[4]  王雨婷, 刘婉滢, 沈舶宁, 赵容, 许亮, 谢明, 康廷国. 黄精的本草考证[J]. 中医药学报, 2019, 47(3): 81–86.

[5]  Wang X, Yang M Y, Shen Y, Zhang Y P, Xiu W Y, Yu S Y, Ma Y Q. Structural characterization and hypoglycemic effect of polysaccharides of Polygonatum sibiricum [J]. Journal of Food Science, 2024, 89(8): 4771–4790.

[6]  王懿文, 周映君, 朱作华, 严理, 胡镇修, 彭源德, 谢纯良. 不同菌株发酵黄精抗氧化、降血糖和降血脂活性比较研究[J]. 上海理工大学学报, 2024, 46(4): 375–381.

[7]  Wang Y T, Niu H M, Ma Y, Yuan G X. Isolation, purification, fractionation, and hepatoprotective activity of Polygonatum polysaccharides [J]. Molecules, 2024, 29(5): 1038.

[8] 孙芸芸, 赵明, 秦诗越, 郑斯纯, 张皓翔, 马晓丹, 李肖梁, 徐伟. 黄精多糖的分离纯化及其免疫调节活性研究[J]. 中国免疫学, 2025, 41(1): 182–188.

[9]  杨庆瑞, 陈婧, 田彩云, 纪佳琦, 郭锁成, 李宁, 牛溢晨, 李吉琪, 高博闻. 不同来源黄精的化学成分分析[J]. 人参研究, 2025, 37(4): 34–36.

[10]  Pu W T, Yu Y F, Shi X X, Shao Y, Ye B H, Chen Y W, Song Q Y, Shen J J, Li H B. The Effect of seasonal and annual variation on the quality of Polygonatum cyrtonema Hua Rhizomes [J]. Plants (Basel), 2024, 13(24): 3459.

[11]  Zhao X, Patil S, Qian A, Qian A R, Zhao C H. Bioactive Compounds of Polygonatum sibiricum–The rapeutic effect and biological activity [J]. Endocr Metab Immune Disord Drug Targets, 2022, 22(1): 26–37.

[12]  林琳, 林寿全. 黄精属药用植物聚类分析[J]. 中药材, 1994, 71: 12–18.

[13]  Meng Y, Nie Z L, Deng T, Wen J, Yang Y P. Phylogenetics and evolution of phyllotaxy in the Solomon’s seal genus Polygonatum (Asparagaceae: Polygonateae): Phylogenetics of Polygonatum [J]. Botanical Journal of the Linnean Society, 2014, 176(4): 435–451.

[14]  Zhao L H, Zhou S D, He X J, Wang Z X, Peng L. A cytotaxonomic analysis of Chinese Polygonatum (Asparagaceae) species [J]. Nordic Journal of Botany, 2014, 32(4): 441–451.

[15]  杨培, 周红, 辛天怡, 马双姣, 段宝忠, 姚辉. 黄精属药用植物DNA条形码鉴定研究[J]. 世界中医药, 2015, 10(8): 1173–1176.

[16]  胡晓燕, 王元忠. 基于傅里叶变换中红外光谱的不同维度光谱图像结合残差神经网络鉴别黄精属物种[J]. 分析测试学报, 2024, 43(11): 1709–1724.

[17]  刘贺贺, 石洪峰. 黄精属植物种质资源遗传多样性研究进展[J]. 现代农业科技, 2023, 3(17): 83–89.

[18]  吴书奇, 谭康瑜, 胡明举, 丛琨, 石瑶, 石亚娜, 董志渊, 杨丽英. 滇黄精及近缘种遗传多样性分析[J]. 分子植物育种. [2025–09–25].

[19]  Chen S L, Yao H, Han J P, Liu C., Song J Y, Shi L C, Zhu Y J, Ma X Y, Gao T, Pang X H, Luo K, Li Y, Li X W, Jia X C, Lin Y L, Leon C. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species [J]. PLoS One, 2010, 5(1): e8613.

[20]  Jiao J, Huang W L, Bai Z Q, Liu F, Ma C D, Liang Z S. DNA barcoding for the efficient and accurate identification of medicinal polygonati rhizoma in China [J]. PLoS One, 2018, 13(7): e0201015.

[21]  王川易, 郭宝林. 植物核基因组核糖体基因间隔区序列的结构特点及其在系统发育研究中的应用[J]. 武汉植物学研究, 2008, 26(4): 417–423.

[22]  Markos S, Baldwin B G. Higher-level relationships and major lineages of Lessingia (Compositae, Astereae) based on nuclear rDNA internal and external transcribed spacer (ITS and ETS) sequences [J]. Systematic Botany, 2001, 26(1): 168–183.

[23] Nuzhdina N, Hassanzadeh A G, Dorogina O. DNA specificity in two Hedysarum sections, Hedysarum and Multicaulia (Siberia, Russia) inferred from ETS sequence data[C] //BIO Web of Conferences. EDP Sciences, 2021, 38: 00092.

[24]  邓菁, 张甜甜, 王迪, 金海珠, 王洪涛. 利用外转录间隔区对中国人参和西洋参进行分子鉴定[J]. 食品与生物技术学报, 2015, 34(4): 420–423.

[25]  Galbany C M, Unwin M, Garcia J N, Smissen R D, Susanna A, Bayer R J. Phylogenetic relationships in Helichrysum (Compositae: Gnaphalieae) and related genera: Incongruence between nuclear and plastid phylogenies, biogeographic and morphological patterns, and implications for generic delimitation [J]. Taxon, 2014, 63(3): 608–624.

[26]  Vander S J, Marant S, Volckaert G. Molecular characterization and phylogenetic utility of the rDNA external transcribed spacer region in Stylosanthes (Fabaceae) [J]. Theoretical and Applied Genetics, 2003, 107(2): 291–298.

[27]  Lu G Q, Moriyama E N. Vector NTI, a balanced all-in-one sequence analysis suite [J]. Briefings in Bioinformatics, 2004, 5(4): 378–388.

[28]  Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets [J]. Molecular Biology and Evolution, 2016, 33(7): 1870–1874.

[29]  龙炳宏, 蒋向辉, 宋荣, 李胜华, 肖龙骞, 易自力, 佘朝文. DNA条形码在黄精属药用植物鉴定与遗传多样性分析中的应用[J]. 植物科学学报, 2022, 40(4): 533–543.

[30]  Fu N, Xu Y, Jin L, Xiao T W, Song F, Yan H F, Chen Y S, Ge X J. Testing plastomes and nuclear ribosomal DNA sequences as the next-generation DNA barcodes for species identification and phylogenetic analysis in Acer [J]. BMC Plant Biology, 2024, 24(1): 445.

[31]  杨森, 左可伟, 张晓娟, 张辰露. 基于SCoT和ISSR分子标记的不同产地黄精遗传分析[J]. 分子植物育种. [2025–09–28].

[32]  吴世安, 吕海亮, 杨继, 饶广远, 尤瑞麟, 葛颂, 钟扬. 叶绿体DNA片段的RFLP分析在黄精族系统学研究中的应用[J]. 植物分类学报, 2000, 58 (2): 97–110.

[33]  Liu H, Cheng H, Xu J, Hu J Y, Zhao C C, Xing L H, Wang M J, Wu Z D, Peng D Y, Yu N J, Liu J L. Genetic diversity and population structure of Polygonatum cyrtonema Hua in China using SSR markers [J]. PLoS One, 2023, 18(8): e0290605.

[34]  Zhang X X, Liu B G, Li Y, Liu Y, He Y X, Qian Z H, Li J X. Landscape genetics reveals that adaptive genetic divergence in Pinus bungeana (Pinaceae) is driven by environmental variables relating to ecological habitats [J]. BMC Ecology and Evolution, 2019, 19(1): 160.

[35]  Pan G, Xie J, Qin Y H, Zhang S H. Development of SSR markers for genetic diversity analysis and species identification in Polygonatum odoratum (Mill.) Druce based on transcriptome sequences [J]. PLos One, 2024, 19(9): e0308316.

[36]  Moritz C. Defining ‘Evolutionarily significant units’ for conservation [J]. Trends in Ecology & Evolution, 1994, 9(10): 373–375.

Options
Outlines

/

〈 〉

Copyright © Editorial office of Subtropical Plant Science
Tel: 0592-5654157　E-mail: yrdzwkx@126.com
Supported by Beijing Magtech Co., Ltd.