The effects of Ginkgo growth was studied under the coupling treatment of acid and aluminum (Al) by applying U₂₀₇ even experimental design and soilless culture. The results showed that Ginkgo could grow normally at the concentration of Al³⁺ of 0.4 mmol/L and below in the range of pH 4.5～5.5, while it could be damaged under the high concentration of Al³⁺ within 0.8~1.2 mmol/L. On other hand, different concentrations of Al³⁺ caused no effect on Ginkgo growth when the pH was higher than 5.0. In a word, Ginkgo growth would be affected especially root system by the coupling treatment of high concentration of Al³⁺ and low pH with the roots got dark and the new roots reduced, even died or rotted, and the plantlet would stop to grow.