The techniques of tissue culture and in vitro conservation of Fosbergia shweliensis were studied. The results showed the aseptic plantlets could be obtained from stem tip of seedlings and stem node explants of F. shweliensis. Their suitable initial medium was MS medium with 3.0 mg·L-1 6-BA, 0.1 mg·L-1 NAA and 3% sucrose, and MS medium with 2.0 mg·L-1 6-BA, 0.1 mg·L-1 NAA and 3% sucrose, respectively. The multiplication rate was up to 3—4 times. The best rooting for plantlets occurred on 1/2MS medium with 1.0 mg·L-1 IBA and 2% sucrose, the rooting rate was 100%. The survival rate of aseptic plantlets transplanted after hardening was more than 90%. The tube-test plantlets could grow slowly on 1/2MS medium with 3% sucrose at 12—15 ℃, in consequence subculture period was lengthened to 18—24 months. The success of rapid propagation and in vitro conservation supported the further research, development and utilization of this species.
LI Ai-hua
,
CHENG Zhi-ying
,
YANG Xiang-yun
. Rapid Propagation and in vitro Conservation of Fosbergia shweliensis[J]. Subtropical Plant Science, 2014
, 43(03)
: 262
-263
.
DOI: 10.3969/j.issn.1009-7791.2014.03.018
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