According to the specific loop-mediated isothermal amplification (LAMP) primers, two specific LAMP methods for the detection of the transgenic soybean GTS40-3-2 strain were established: (1) real-time fluorescence loop-mediated isothermal amplification (RT-LAMP); (2) LAMP with hydroxy naphthol blue (HNB) dye as indicator. The specificity and sensitivity of these two methods were evaluated. The results indicated that the transgenic soybean GTS40-3-2 could be distinguished from several GM events and non-GM plants with limitation of dection of 0.1%. The LAMP assay with HNB dye requires only simple laboratory equipment and it is suitable for daily inspection and supervision departments for rapid detection of genetically modified ingredients.
YANG Li-Xia
,
QIU Hua-Li
,
PENG Xin-Kai
,
SONG Tao-Ping
. Establishment of Loop-mediated Isothermal Amplification Method for Detection of Transgenic Soybean GTS40-3-2 Strain[J]. Subtropical Plant Science, 2015
, 44(03)
: 213
-217
.
DOI: 10.3969/j.issn.1009-7791.2015.03.007
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