Inhibitory Activity of Antibacterial and Quorum Sensing of Water-soluble Polysaccharide from Phyllanthus emblica Fruit

  • CAO Li-li ,
  • WANG Fang ,
  • YANG He-zhong ,
  • LIAO Zheng-ping ,
  • WU Shao-hua ,
  • LI Yong-yu
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  • (College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China)

Received date: 2015-11-18

  Revised date: 2016-01-15

  Online published: 2015-12-10

Abstract

The inhibitory activities of bacteria and quorum sensing of water-soluble polysaccharide from Phyllanthus emblica fruit were studied. The activity of antibacterial to 5 tested bacterials and the effect on violacein yield of Chromobacterium violaceum CV026 were carried out using turbidimetry. An order of the inhibitory activities of polysaccharide to 5 tested bacteria was Staphylococcus aureus ATCC25933 > Bacillus subtilis ATCC21216 > C. violaceum CV026 > S. marcescens H30 > Escherichia coli ATCC25922; Violacein yield of C. violaceum CV026 was inhibited strongest (53.47?3.10)% at the concentration of 20 mg穖L-1 emblica polysaccharide. In conclusion, the water-soluble polysaccharide of emblica has inhibitory effect of bacterial multiplication and quorum sensing.

Cite this article

CAO Li-li , WANG Fang , YANG He-zhong , LIAO Zheng-ping , WU Shao-hua , LI Yong-yu . Inhibitory Activity of Antibacterial and Quorum Sensing of Water-soluble Polysaccharide from Phyllanthus emblica Fruit[J]. Subtropical Plant Science, 2015 , 44(04) : 289 -292 . DOI: 10.3969/j.issn.1009-7791.2015.04.005

References

[1] 周涛,邱德文. 民族药余甘子的本草学概况[J]. 贵阳中医学院学报, 2002,24(3): 3—5.

[2] 李永裕. 余甘多糖分离纯化、结构及抗氧化活性研究[D]. 福州: 福建农林大学博士学位论文, 2010.

[3] 郑宝东,曾绍校. 余甘多糖对小鼠的抗氧化作用[J]. 福建农林大学学报(自然科学版), 2005,33(1): 110—112.

[4] Liu X L, Cui C, Zhao M M, Wang J S, Luo W, Yang B, Jiang Y M. Identification of phenolics in the fruit of emblica (Phyllanthus emblica L.) and their antioxidant activities[J]. Food Chemistry, 2008,9(4): 909—915.

[5] 侯开卫,刘凤书,李绍家,杨臣武,赵苹. 余甘果抗衰老作用的研究[J]. 食品科学, 1990,4(2): 2—5.

[6] 罗春丽. 余甘子对肿瘤细胞抑制作用及免疫调节的研究[J]. 中国实验方剂学杂志, 2010(13): 155—158.

[7] Qi W Y, Li Y, Hua L, Wang K, Gao K. Cytotoxicity and structure activity relationships of phytosterol from Phyllanthus emblica[J]. Fitoterapia, 2013,84: 252—256.

[8] Liu X L, Zhao M M, Wu K, Chai X H, Yu H P, Tao Z H, Wang J S. Immunomodulatory and anticancer activities of phenolics from emblica fruit (Phyllanthus emblica L.)[J]. Food Chemistry, 2012,31(2): 685—690.

[9] Zhong Z G, Wu D P, Huang J L, Liang H, Pan Z H, Zhang W Y, Lu H M. Progallin A isolated from the acetic ether part of the leaves of Phyllanthus emblica L. induces apoptosis of human hepatocellular carcinoma BEL-7404 cells by up-regulation of Bax expression and down-regulation of Bcl-2 expression[J]. Journal of Ethnopharmacology, 2011,133(2): 765—772.

[10] Fatima N, Pingali U, Muralidhar N. Study of pharmacodynamic interaction of Phyllanthus emblica extract with clopidogrel and ecosprin in patients with type II diabetes mellitus[J]. Phytomedicine, 2014,21(5): 579—585.

[11] 康文娟,张广梅,赵协慧,童丽,王津慧,刘占厚. 不同溶剂余甘子提取物的降血糖作用研究[J]. 安徽农业科学, 2011,39(30): 18 545—18 547.

[12] 胡炜. 余甘子提取物对糖尿病大鼠肌肉和脂肪组织胰岛素信号通路的影响[J]. 中国组织工程研究, 2012,16(11): 2007—2010.

[13] Lee C Y, Peng W H, Cheng H Y, Chen F N, Lai M T. Hepatoprotective effect of Phyllanthus in Taiwan on acute liver damage induced by carbon tetrachloride[J]. The American Journal of Chinese Medicine, 2006,34(3): 471—482.

[14] 高伦江,陈岗,唐春红,曾顺德. 余甘子有效抑菌成分提取工艺优化[J]. 食品工业科技, 2012,33(18): 279—281.

[15] Perianayagam J B, Sharma S, Joseph A, Chistina A J M. Evaluation of anti-pyretic and analgesic activity of Emblica officinalis Gaertn[J]. Journal of Ethnopharmacology, 2004,95(1): 83—85.

[16] Sripanidkulchai B, Junlatat J. Bioactivities of alcohol based extracts of Phyllanthus emblica branches: antioxidation, antimelanogenesis and anti-inflammation[J]. Journal of Natural Medicines, 2014,68(3): 615—622.

[17] 黄清松,曾满红,李红枝,郑敏,郭静波,潘育方. 余甘子果汁饮料抗疲劳及抗衰老功效研究[J]. 南方农业学报, 2014,45(5): 840—843.

[18] 刘春兰,杨逸,何林,周宜君. 植物多糖抑菌作用研究方法进展[J]. 时珍国医国药, 2013,24(7): 1725—1727.

[19] 李斌. 黑木耳黑色素对细菌群体感应调控行为的抑制及其抗氧化活性的研究[D]. 南京: 南京农业大学博士学位论文, 2011.

[20] 王卉,杨梦华,钟增涛,朱军. 人类病原细菌中的群体感应[J]. 医学分子生物学杂志, 2006,3(2): 146—148.


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