The optimum extraction conditions of tenacissima G from Marsdenia tenacissima were investigated. On the basis of single factor test, the alcohol concentration, solid-liquid ratio, extraction duration and extraction times were selected for the four-factor, three-level Box-Behnken experimental design. In addition, Design Expect 8.0 software was employed to analyze the test data and the response surface methodology was applied to optimize the extraction conditions. The results suggested that optimum extraction conditions consisted of 80% ethanol as the extraction solvent, an extraction duration of 1.5 h, a solid-liquid ratio of 1∶20(W/V) and extraction of two times. Under these optimum conditions, the extraction yields of tenacissima G from M. tenacissima was 0.253%.
WANG Ze-feng
,
SHI Ling
,
ZHANG Wei
,
XU Chun
,
ZHANG Li
,
MIN Yong
. Optimization of Extraction Process for Tenacissima G from Marsdenia by Response Surface Methodology[J]. Subtropical Plant Science, 2016
, 45(01)
: 7
-12
.
DOI: 10.3969/j.issn.1009-7791.2016.01.002
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