Characteristics and Expression of Dormancy-Associated MADS-Box PpMADS13 in Different Chilling Requirement Pear Cultivars

  • LI Liang ,
  • LIU Hang ,
  • ZHANG Gui-chi ,
  • WU Shao-hua ,
  • LI Yong-yu
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  • (College of Horticulture, Fujian Agriculture and Forestry University; Institute of Natural Products of Horticultural Plants, Fuzhou 350002, Fujian China)

Received date: 2015-12-08

  Revised date: 2016-04-17

  Online published: 2016-03-10

Abstract

The buds from ‘Huanghua’ and ‘Mixue’, two cultivars of Pyrus pyrifolia, were used as the experiment materials to explore the characteristics and expression of dormancy-associated MADS-box (DAM) genes PpMADS13-1 and PpMADS13-3 in different chilling requirement pear cultivars from Fujian. PpMADS13 genes were cloned and used the QRT-PCR to analysis their expression changes during the dormancy period. The results showed that PpMADS13-1 was conservative in low chilling requirement pears. The conservatism of PpMADS13-3 was not so high in different chilling requirement pears. The PpMADS13-1hh and PpMADS13-3hh in ‘Huanghua’ were gradual down-regulation with dormancy release. The PpMADS13-1mx and PpMADS13-3mx in ‘Mixue’ presented a trend of ‘M’ type during the dormancy. The result indicated that both PpMADS13-1 and PpMADS13-3 work in dormancy release, but their regulation model were different between two pear cultivars.

Cite this article

LI Liang , LIU Hang , ZHANG Gui-chi , WU Shao-hua , LI Yong-yu . Characteristics and Expression of Dormancy-Associated MADS-Box PpMADS13 in Different Chilling Requirement Pear Cultivars[J]. Subtropical Plant Science, 2016 , 45(01) : 42 -47 . DOI: 10.3969/j.issn.1009-7791.2016.01.009

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