Subtropical Plant Science >

2016 , Vol. 45 >Issue 01: 48 - 52

DOI: https://doi.org/10.3969/j.issn.1009-7791.2016.01.010

Construction and Identification of Flower Buds cDNA Library of Asian Lotus(Nelumbo nucifera)

  • WU Xuan ,
  • ZHANG Da-sheng ,
  • TIAN Dai-ke ,
  • ZHANG Wei-wei ,
  • LIU Qing-qing ,
  • LIU Feng-luan ,
  • CHEN Qing ,
  • ZHAO Xi-shuang ,
  • WU Shao-hua
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  • (1.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 2.Shanghai Chenshan Plant Science Research Center, Shanghai Chenshan Botanical Garden, Chinese Academy of Sciences, Shanghai 201602, China; 3.Shanghai Key Laboratory for Plant Functional Genomics and Resources, Shanghai 201602, China; 4.Agriculture and Forestry Gardening, Shanghai Vocational College, Shanghai 201699, China)

Received date: 2016-01-13

  Revised date: 2016-04-17

  Online published: 2016-03-10

Fold

Abstract

To explore the transcriptome during floral organ development in Asian lotus(Nelumbo nucifera), the total RNA of flower buds was isolated from three typical lotus cultivars: N. nucifera ‘Honghu Honglian’(single flower), N. nucifera ‘Tangzhaotisi Lian’(double flower) and N. nucifera ‘Qianban Lian’(fully-doubled and thousand-petalled). The double-stranded cDNA was synthesized based on the extracted mRNA using SMART method, then digested with restriction enzyme SfiI, and finally purified and ligated to the pUC19 vector. Thereafter, the flower buds cDNA library was constructed and amplified. The constructed cDNA library had the titer of 1.12×106 pfu·mL-1. The insert sizes of the library were 500—2000 bp and the recombination efficiency of cDNA library was about 95%. The cDNA library would provide the fundamental information for studying on gene expressions and regulations during floral organ development in lotus flower buds.

Key words： lotus; flower buds; cDNA library

Cite this article

WU Xuan , ZHANG Da-sheng , TIAN Dai-ke , ZHANG Wei-wei , LIU Qing-qing , LIU Feng-luan , CHEN Qing , ZHAO Xi-shuang , WU Shao-hua . Construction and Identification of Flower Buds cDNA Library of Asian Lotus(Nelumbo nucifera)[J]. Subtropical Plant Science, 2016 , 45(01) : 48 -52 . DOI: 10.3969/j.issn.1009-7791.2016.01.010

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