In this study, total RNA extraction of roots, stems, and leaves from Cinnamomum kanehirae was carried out. Electrophoresis, Nanodrop and Aglient 2100 analysis indicated that the value of 28S/18S was 1.7 to 2.1, RNA?integrity number was higher than 7, and RNA concentration was higher than 50 ng·μL-1, indicating that RNA was intact. In summary, the study showed that the purity, concentration and?integrity of the isolated RNA were qualified, and could be used for subsequent molecular biology studies.
MENG Hong-yan
,
GUO Ying
,
LIN Wen-zhen
,
WANG Wen-hua
,
LIU Li-qing
. A Method for Extraction of Total RNA from Cinnamomum kanehirae[J]. Subtropical Plant Science, 2016
, 45(01)
: 53
-56
.
DOI: 10.3969/j.issn.1009-7791.2016.01.011
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