To make use of plant fruit specific express foreign proteins, 1.2 kb AGPL1 gene promoter was amplified in watermelon leaves using PCR, and bioinformatics analysis of AGPL1 promoter sequences was conducted. The results showed by PlantCARE database and PLACE database that AGPL1 gene promoter had number of typical core elements such as TATA-Box, CAAT-Box, high level transcription cis-acting element 5UTR Py-rich stretch, photoresponse elements including ACE, ATCC-motif, Box 4, I-Box, Sp1, TCCC-motif, GAG-motif, MNF1, two gibberellin response elements GARE-motif and P-box, cis-acting element related to protein metabolic regulation O2-site, cis-acting element related to salicylic acid reaction TCA-element, induced anaerocabically cis-acting element ARE, element involved in defense and stress responsiveness TC-rich repeats, circadian rhythm controlling element circadian and so on, all of which lays the groundwork for such research on regulation of PG genes in the future.
ZHOU Li-ying
,
BU Lu-lu
,
YANG Chun-lei
,
GAO Ting
,
TIAN Tian
,
YANG Zheng-an
. Cloning and Sequence Analysis of AGPL1 Promoters in Watermelon Fruit Tissue[J]. Subtropical Plant Science, 2016
, 45(02)
: 122
-126
.
DOI: 10.3969/j.issn.1009-7791.2016.02.005
[1] 路静,赵华燕,何奕昆,宋艳茹. 高等植物启动子及其应用研究进展[J]. 自然科学进展, 2004(8): 17—23.
[2] 糜赛男,曹明富. 植物组织特异性启动子的研究进展[J]. 生物学教学, 2010,35: 7.
[3] 周丽英,杨正安. 番茄PG基因启动子的序列分析[J]. 北方园艺, 2016(1): 72—75.
[4] 尹涛,张上隆,刘敬梅,陈大明. 果实特异性启动子研究现状及其应用[J]. 农业生物技术学报, 2009(2): 355—360.
[5] Fickett J W, Haziigeorgiou A G. Eukaryotic promoter recognition[J]. Genome Research, 1997,7: 861—878.
[6] 皮灿辉,易自力,王志成. 提高转基因植物外源基因表达效率的途径[J]. 中国生物工程杂志, 2003,23(1): 1—4.
[7] 王志新,赵琳,李文滨. 植物诱导型启动子的研究进展[J]. 大豆科技, 2011(3): 5—9.
[8] 贺红霞,陈亮,林春晶,柳青. 组织特异性启动子在作物基因工程中的研究进展[J]．中国农学通报, 2014,30(9): 225—231.
[9] 王纪. 香蕉果实特异表达启动子的分离鉴定[D]. 海口: 海南大学硕士学位论文, 2010.
[10] 李孟娇. 柑橘果实成熟特异启动子的分离与功能初步分析[D]. 临安: 浙江农林大学硕士学位论文, 2013.
[11] 夏江东,程在全,吴渝生,季鹏章. 高等植物启动子功能和结构研究进展[J]. 云南农业大学学报, 2006(1): 7—14.
[12] 刘敬梅,陈大明,许勇,陈杭. 西瓜果实特异性基因wml1的5′端上游调控序列的分离[J]. 实验生物学报, 2002(1): 31—35.
[13] 吴韩英,刘敬梅,杨信廷,朱祝军,寿森炎. 西瓜果实特异启动子WSP功能区域的初步定位[J]. 生物工程学报, 2003(2): 227—230.
[14] Kim I J, Kang H Y, Chung W I. Characterization of cDNAs encoding small and large subunits of ADP-glucose pyrophpsphorylases from watermelon (Citrulus valgris S.)[J]. Bioscience, Biotechnology, and Biochemistry, 1998,62(3): 550—555.
[15] Montgomery J, Pollard V, Deikman J, Fischer R L. Positive and negative regulatory regions control the spatial distribution of polygalacturonase transcription in tomato fruit pericarp[J]. Plant Cell, 1993,5: 1049—1062.
[16] Koltunow A M, Truettner J, Cox K H, Wallroth M, Goldberg R B. Different temporal and spatial gene expression patterns occur during anther development[J]. Plant Cell, 1990,2: 1201.
[17] 刘玉瑛,张江丽. 几个真核生物启动子计算机预测数据库资源概述[J]. 湖南农业科学, 2007(4): 70—71,74.
[18] 刘玉瑛,张江丽. 真核生物启动子预测相关数据库资源概述[J]. 安徽农业科学,2007,24: 7418—7419.
[19] 胡翔,周建林. 真核生物基因启动子的计算机预测及其软件资源[J]. 激光生物学报, 2004(6): 465—467.
[20] 毛娟,陆璐,陈佰鸿,禇明宇,赵长增. 甜瓜CmACOI启动子组织特异性表达研究[J]. 园艺学报, 2013,40(60): 1101—1109.
[21] 孙芳芳,宋洪元. 植物诱导型启动子研究进展[J]. 南方园艺, 2014,25(2): 51—57.
