Plant physiology, biochemistry and molecular biology

The Evaluation of Antioxidant Activity of Polyphenol Compounds Using ORAC

  • CHANG Qiang ,
  • SU Ming-Hua ,
  • CHEN Qing-Xi ,
  • ZENG Bi-Yu ,
  • WANG Wei ,
  • LI Hui-Hua
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  • (1.College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian China; 2.Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China)

Received date: 2016-08-04

  Revised date: 2016-10-12

  Online published: 2016-09-10

Abstract

The oxygen radical absorbance capacity (ORAC) method was validated, and then ORAC values of selected 20 phenolic compounds were determined. The results indicated that linearity (R2 =0.997), limit of detection and limit of quantification (0.5—3.1 μmol·L-1), precision (<18%), accuracy (91%—105%) were determined. And ellagic acid and gallic acid had strong antioxidant activity in p-hydroxy benzoic acid compounds. Caffeic acid and its derivatives had highest antioxidant activity in hydroxy cinnamic acid. In the flavonoid constituents, flavan-3-ols showed good antioxidant properties, followed by flavonol. ORAC was considered as simple, efficient detection method of standardization for evaluation of antioxidant activity of the phenolic compounds.

Cite this article

CHANG Qiang , SU Ming-Hua , CHEN Qing-Xi , ZENG Bi-Yu , WANG Wei , LI Hui-Hua . The Evaluation of Antioxidant Activity of Polyphenol Compounds Using ORAC[J]. Subtropical Plant Science, 2016 , 45(03) : 225 -229 . DOI: 10.3969/j.issn.1009-7791.2016.03.006

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