Subtropical Plant Science >

2011 , Vol. 40 >Issue 01: 34 - 37

DOI: https://doi.org/10.3969/j.issn.1009-7791.2011.01.009

Tissue Culture of Brassica alboglabra

  • CHEN Qiong-e ,
  • ZHONG Feng-lin ,
  • LIN Bi-ying
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  • 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002;
    2. Vegetable Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002

Received date: 2010-09-30

  Online published: 2011-03-10

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Abstract

This experiment focused on testing the most suitable substrate for the tissue culture of Brassica alboglabra.The caulis segments and hypocotyl were separated from aseptic seedlings and used as explants.The results showed the best culture medium for hypocotyl was MS+6-BA 3.0 mg/L+NAA 0.2 mg/L, and for the caulis segments was MS+6-BA 5.0 mg/L+NAA 0.2 mg/L.The most suitable substrate for multiplication of adventitious buds was MS+6-BA 3.0 mg/L+NAA 0.2 mg/L, and the substrate with 1/2 MS+NAA 0.1 mg/L+IBA 0.2 mg/L had the best performance for rooting culture.The survival percentage of transplanting seedlings reached 96.6%.

Key words： Brassica alboglabra; stem segments; hypocotyl; tissue culture; rapid propagation

Cite this article

CHEN Qiong-e , ZHONG Feng-lin , LIN Bi-ying . Tissue Culture of Brassica alboglabra[J]. Subtropical Plant Science, 2011 , 40(01) : 34 -37 . DOI: 10.3969/j.issn.1009-7791.2011.01.009

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