Through the establishment of tissue culture rapid propagation system, the effects of KT and NAA on the induction of new stem, callus and roots of Astragalus membranceus were studied.The induction of new stem was related to the ratio of KT/NAA, which was obvious with the average number of new stem was the maximum, 11.7, when the value was 4.With the promotion of NAA, lots of white callus was produced.The average diameter of white callus, which was in the medium with 1 mg/L of NAA, was 1.4 cm.Roots came mainly from the white callus, being promoted by NAA but inhibited by KT.1 mg/L of NAA played a key positive role in the increase of roots number, the average length of roots and the number of secondary roots, but negative in roots average diameter.