Adventitious Buds Induction and Rapid Propagation from Leaflet Explants of Arachis hypogaea

  • LIU Can ,
  • SU Liang-chen ,
  • ZHUANG Yi-na ,
  • QIN Ming ,
  • LI Ling
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  • College of Life Science, South China Normal University, Guangzhou 510631, Guangdong China

Received date: 2009-12-31

  Online published: 2010-06-10

Abstract

The leaflet were used as explants to study plant regeneration,and the results showed that the adventitious bud differentiation could be effectively induced from the explants on medium MS+0.6 mg/L NAA+8 mg/L 6-BA+1 mg/L AgNO3+3 mg/L Gln,and shoot induction rate and average number of shoots per explant was 81.03% and 5.79 respectively.The optimum medium for adventitions bud elongation was MS+2 mg/L 6-BA+4 mg/L GA3,and the average elongation length was 1.0-2.0 cm.The rooting rate of explants on 1/2 MS+0.5 mg/L NAA+3 mg/L IBA was 86.15%,and the root was stronger,longer and had more lateral roots.After hardening-off,the survival rate and maturing rate reach to 90% and 100% respectively.

Cite this article

LIU Can , SU Liang-chen , ZHUANG Yi-na , QIN Ming , LI Ling . Adventitious Buds Induction and Rapid Propagation from Leaflet Explants of Arachis hypogaea[J]. Subtropical Plant Science, 2010 , 39(02) : 21 -24,37 . DOI: 10.3969/j.issn.1009-7791.2010.02.006

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