Preliminary Study on Embryo Rescue of Interspecific Hybrids between Cymbidium

  • ZENG Bi-yu ,
  • XU Chuan-jun ,
  • XU Su-xia ,
  • ZHANG Wen-hui ,
  • HUANG Qing-yun
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  • Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China

Received date: 2010-06-07

  Online published: 2010-09-10

Abstract

Interspecific hybridization was carried out with Cymbidium hybridium as female parent and C. sinense as male parent.Embryo rescue technique of hybrids was also studied.The results showed that ovaries culture was better than ovules culture, and ovaries culture had become to be effective means of embryo rescue.The germination and growth had significant difference at different growth stages and different culture medium.Ovaries could be cultured in vitro successfully as early as 60d after pollination.However, the germination rate was the highest when ovaries cultured for 90d after pollination, which reached up to 80%.Of the media studied, VW+BA 0.2 mg/L+NAA 0.2 mg/L+peptone 3 g/L+cascin hydrolyaate 500 mg/L+coconut milk 10% was the optimal medium for ovaries culture.

Cite this article

ZENG Bi-yu , XU Chuan-jun , XU Su-xia , ZHANG Wen-hui , HUANG Qing-yun . Preliminary Study on Embryo Rescue of Interspecific Hybrids between Cymbidium[J]. Subtropical Plant Science, 2010 , 39(03) : 12 -14 . DOI: 10.3969/j.issn.1009-7791.2010.03.004

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