Subtropical Plant Science >

2010 , Vol. 39 >Issue 04: 4 - 6

DOI: https://doi.org/10.3969/j.issn.1009-7791.2010.04.002

Prokaryotic Expression,Purification and Polyclonal Antibody Preparation of Peanut AhAO1 Protein

  • YANG Li-xia ,
  • SONG Tao-ping ,
  • PENG Xin-kai ,
  • HU Zhao-hui ,
  • LI Ling
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  • 1. Changsha Center of Supervision & Inspection on Food Quality Safety, Changsha 410013, Hunan China;
    2. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, Guangdong China

Received date: 2010-06-28

  Online published: 2010-12-10

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Abstract

The AhAO1 gene was isolated by RT-PCR using cDNA synthesized from total RNA extracted from peanut leaves and cloned into plasmid pPROEXHTa.The expression plasmid HTaAhAO1 was transformed into E.coli BL21.SDS-PAGE analysis revealed that the His-AhAO1 fusion protein was highly expressed in E.coli BL21 and purified by affinity chromatography and electrophoresis.The His-AhAO1 polyclonal antibody was prepared and tested by western blot.The anti-His-AhAO1 polyclonal antibody lays foundation for further research on AhAO1.

Key words： Arachis hypogaea; AhAO1; pPROEXHTa; prokaryotic expression; antibody

Cite this article

YANG Li-xia , SONG Tao-ping , PENG Xin-kai , HU Zhao-hui , LI Ling . Prokaryotic Expression,Purification and Polyclonal Antibody Preparation of Peanut AhAO1 Protein[J]. Subtropical Plant Science, 2010 , 39(04) : 4 -6 . DOI: 10.3969/j.issn.1009-7791.2010.04.002

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