Subtropical Plant Science >

2009 , Vol. 38 >Issue 02: 15 - 18

DOI: https://doi.org/10.3969/j.issn.1009-7791.2009.02.004

Influences of PPO Inhibitors on Browning in Phalaenopsis Explants

  • CHEN Dong-yin ,
  • LAI Yan-yan ,
  • XU Chuan-jun ,
  • LI Ling
Expand
  • 1. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, Guangdong China;
    2. Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China

Received date: 2009-01-19

  Online published: 2009-06-10

Fold

Abstract

The effects of Polyphenol oxidase (PPO) activity inhibitors supplemented to reaction buffer at different concentrations showed that ascorbate acid and Cys complete inhibited PPO activity at 0.5 mmol/L. Adding PPO activity inhibitors to media at different concentrations, the results showed that 300 mg/L citric acid and 100-200mg/L NaHSO3 could reduce the browning of Phalaenopsis explants. Soaking explants with PPO activity inhibitors, it showed that 50 mg/L ascorbate acid gave the best result for browning inhibition and PPO activity was lower than that of control before explants browning happened.

Key words： Phalaenopsis; explants; browning; polyphenol oxidase; inhibitor

Cite this article

CHEN Dong-yin , LAI Yan-yan , XU Chuan-jun , LI Ling . Influences of PPO Inhibitors on Browning in Phalaenopsis Explants[J]. Subtropical Plant Science, 2009 , 38(02) : 15 -18 . DOI: 10.3969/j.issn.1009-7791.2009.02.004

Options
Outlines

/

〈 〉

Copyright © Editorial office of Subtropical Plant Science
Tel: 0592-5654157　E-mail: yrdzwkx@126.com
Supported by Beijing Magtech Co., Ltd.