Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro

  • GAO Li ,
  • YANG Bo ,
  • LI Hong-lin
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  • Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei China

Received date: 2008-12-03

  Online published: 2009-06-10

Abstract

Stems of tissue culture seedlings of Ormosia henryi were cultured under low temperature stress. The results showed that the optimal temperature for induction of chilling tolerance callus was 5℃. The differentiation frequency of chilling tolerance callus cultured under 5℃ was 70.0% on MS solid medium supplemented with 6-BA 2.0 mg/L and NAA 0.25 mg/L, and the injury rate of regenerated plants from chilling tolerant callus was much lower than that of control group.

Cite this article

GAO Li , YANG Bo , LI Hong-lin . Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro[J]. Subtropical Plant Science, 2009 , 38(02) : 19 -21,25 . DOI: 10.3969/j.issn.1009-7791.2009.02.005

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