Based on the reported Cymbidium mosaic virus (CyMV) genomic cDNA sequence, specific primer of CymV-CP was designed, and RT-PCR was performed with the total RNA as templates extracted from diseased orchid plant collected from Xiamen, to attain complete CP gene sequence. The result was that the virus isolates of XM orchid had high homology with isolates that separated form other areas of China and Asian countries (the homologies were all above 96% at nuclear acid). According to phylogenetic tree analysis, the virus isolates of XM orchid clustered with reference sequences of Taiwan, Korea and India, and Fujian, Beijing and Singapore isolates clustered together.
GUO Ying
,
LIN Zhi-kai
,
LIU Li-qing
,
LIN Qing-hong
. Molecular Identification of Cymbidium mosaic Virus Infecting Orchid in Xiamen[J]. Subtropical Plant Science, 2011
, 40(03)
: 1
-3
.
DOI: 10.3969/j.issn.1009-7791.2011.03.001
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