The C-serum is a major component of the latex and a key material for proteinomics of Hevea brasiliensis. Using the 2-D electrophoresis, there are some disadvantages with protein extraction from C-serum, such as unsatisfied solubility, higher ions concentrations and too many impurities. In this paper, the alcohol-ether method and sonication were used to further remove interfering substances and solubilized protein based on the traditional method of TCA/acetone. Comparing the traditional method of extraction, the results showed that the new method improved the solubility, ions concentration and purity of protein samples from C-serum. The optimized methods for extraction of protein will be extremely useful for the proteomic research of C-serum form H. brasiliensis latex.
QI Ji-yan
,
LAN Ji-xian
,
QIN Yun-xia
,
LONG Xiang-yu
. Optimization of Protein Preparation Suitable for 2-DE of Hevea brasiliensis C-serum[J]. Subtropical Plant Science, 2012
, 41(03)
: 16
-20
.
DOI: 10.3969/j.issn.1009-7791.2012.03.004
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