Mesophyll protoplasts were isolated and purified from leaves of in vitro propagated Polgostemon cablin (Blanco) Benth. cv. shipaiensis, and different factors affecting protoplasts yield and activity were investigated. The results showed that young leaves of seedling subcultured for 12～22 days in the enzyme solution consisting of 0.5% (w/v) pectolyase Y-23, 0.2%(w/v) Macerozyme R-10 and 1. 5% (w/v) cellulase R-10, in 11% (w/v) mannitol buffered with 0.1% MES and 0.02% CaCl2 for 8 h, the yield was 1.85×107 protoplasts per gram leaves (fresh weight), the viability was above 89%.
MO Xiao-lu
,
QIU Wei-fen
,
HUANG Shan-shan
,
CHEN Yu-zhen
,
YAN Zhen
. Isolation and Purification of Mesophyll Protoplasts of Pogostem cablin cv. shipaiensis[J]. Subtropical Plant Science, 2012
, 41(03)
: 25
-28
.
DOI: 10.3969/j.issn.1009-7791.2012.03.006
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