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10 June 2008, Volume 37 Issue 02 Previous Issue    Next Issue

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Dynamics of Protein during Flower-Bud Formation in Eriobotrya japonica LIU Zong-li, LIN Shun-quan, CHEN Hou-bin 2008, 37(02): 1-6.  Abstract ( )   PDF (1476KB) ( )   The variation of protein in the leaves during flower-bud formation in potted and field-grown ‘Zaozhong’ loquat trees were studied. During flower induction, soluble protein content of leaves on floral shoots rose, while during flower differentiation, it fell sharply. Flower induction and differentiation were two distinct phases related with each other. The two-electrophoresis diagrams of protein extracted from floral buds and vegetative buds were on the whole similar to each other. However, in the diagram from floral buds there were two proteins of iso-electric point (pI) and molecular weight (MW) of pI 5.350±0.008, MW 14110.5±110.8 and pI 4.730±0.032, MW 66446.3±260.9, respectively, which didn't exist in vegetative buds. Because the two new proteins were acidic, i.e. soluble proteins, they were maybe closely related with flowering in loquat. Related Articles | Metrics

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Floral Organogenesis of Manglietia fordiana Oliv. and Manglietia chingii Dandy LIAO Wen-fang, XIA Nian-he 2008, 37(02): 7-10.  Abstract ( )   PDF (1574KB) ( )   The floral organogenesis of Manglietia chingii Dandy and Manglietia fordiana Oliv. were observed under scanning electron microscope (SEM). Tepals of Manglietia chingii and Manglietia fordiana initiate asynchronously in whorls. Tepals of Manglietia fordiana are arranged in 3 whorls with the number of 3. Tepals of Manglietia chingii are arranged in 4 whorls, and degenerative tepals in the fourth whorl can be observed frequently. Stamens and carpels of Manglietia fordiana and Manglietia chingii all arise spirally and in acropetal succession. Related Articles | Metrics

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Study on Leaf Chlorophyll Content of 57 Evergreen Tree Species Grown in Mountain Meihuashan, Fujian Province YE Qian, LIU Zhi-guo, CAI Yong-li, CHEN Ling, WANG Hong-wei, TIAN Yu-peng 2008, 37(02): 11-16.  Abstract ( )   PDF (3970KB) ( )   The leaf chlorophyll content and Chla, Chlb, Chla/b of 57 evergreen tree species grown at two altitudes (1 200m and 455m) in Meihuashan National Nature Reserve were investigated. The main results were shown as follows:The values of Chla, Chlb, Chla+b and Chla/b of these trees distributed in a centralized region. The chlorophyll content of new leaves is higher than that of old leaves, Chla/b of new leaves is lower than that of old leaves. The chlorophyll content and Chla/b of tree leaves tended to decrease with the increasing altitude, and the trees grown at different altitudes (1 200m and 455m) had obvious differences. The photosynthesis characteristics of plants changed with the environment they grown. Related Articles | Metrics

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Optimization of Agrobacterium-mediated Transformation System for Torenia fournieri LONG Hai-tao, LI Hong-qing, LI Ling 2008, 37(02): 17-20.  Abstract ( )   PDF (1000KB) ( )   An efficient and stable Agrobacterium-mediated transformation system for Torenia was established through investigating the factors influencing the transformation efficiency. Genetic transformation of Torenia was performed by using 5~6 week leaflet as explants. After incubation in Agrobacterium suspension of OD600 at 0.5~0.6 for 5~10min, the explants were co-cultivated on co-culture medium containing 200μmol/L acetosyringone for 4d under dark. The explants were then transferred to the calla induction agar medium MS+BA 1.0mg/L+2,4-D 0.1mg/L, and cultivated for 14d to obtain the resistant calla. The resistant calla were transferred to the shoot induction agar medium 1/2MS+BA 1.0mg/L+NAA 0.1mg/L, and cultured for another 28d to obtain resistant shoots. The 0.5cm or taller resistant shoots were finally transferred to rooting agar medium, and the resistant plantlets were achieved after 14d cultivation. Hygromycin-resistant plants were confirmed to be positive transformants by PCR. The transformation frequency was up to 13%~14% overall. Related Articles | Metrics

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Studies of Dedifferentiation and Redifferentiation of Pueraria lobata Earthnut YIN Ming-hua, HONG Sen-rong 2008, 37(02): 21-25.  Abstract ( )   PDF (923KB) ( )   The effects of several factors on dedifferentiation and redifferentiation of Pueraria lobata earthnut were investigated by orthogonal experiment, single factor experiment and plant tissue culture. The results showed that the best culture medium of Pueraria lobata earthnut callus induction was MS+NAA 0.5 mg/L+6-BA 1.0 mg/L+2,4-D 2.0 mg/L and culture in dark was more advantageous to Pueraria lobata earthnut callus induction. The best culture medium of Pueraria lobata earthnut callus redifferentiation was MS+NAA 0.5 mg/L+6-BA 3.0 mg/L or MS+NAA 0.5 mg/L+KT 2.0 mg/L, and culture in light was more advantageous to the callus redifferentiation. The best culture medium of Pueraria lobata earthnut callus regenerated buds rooting is MS+NAA 0.5 mg/L+PP333 3.0 mg/L+30 g/L sucrose. 3.0 mg/L PP333 and transplant matrix of vermiculite:perlite (2:1) could remarkably improve the transplant survival rate of the regenerated seedling. Related Articles | Metrics

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RAPD Analysis of 14 Wampee Cultivars JIANG Su-mei, FENG Li, LIN De-qiu 2008, 37(02): 26-29.  Abstract ( )   PDF (1042KB) ( )   Fourteen wampee cultivars collected from Guangdong and Guangxi were used for RAPD analysis in this study. 16 primers selected from 150 random primers were used to amplify 87 DNA fragments and 57 bands (54.0%) showed polymorphisms. Genetic distance data showed that the heriditary conservation among 11of 14 studied cultivars (except Longshan akaryotic wampee, Longchuan akaryotic wampee and white sugar karyotic wampee) was very high (D≤0.1), which suggested that the genetic relationship between theses cultivars was very close. Based on UPGMA analysis, these 14 cultivars were divided into three groups at the level of D=0.15, which was not fully consistent with the morphological traits of these cultivars. Related Articles | Metrics

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Screening Oxalate Oxidase from Plants and Evaluation on Their Application DONG Deng-feng, WANG Yong-feng 2008, 37(02): 30-34.  Abstract ( )   PDF (921KB) ( )   88 plant species from 44 families were used as raw material to screen oxalate oxidase (OXO), from which, 60 species from 34 families showed detectable OXO activities, and 30 species showed quite high OXO activity amounting to >0.02 U/mg protein, and >0.2 U/g·fw. In order to evaluate the possibility of scavenging oxalate in mammalian body by OXO, five plant species with high biomass and OXO activity, namely, Echinacea purpurea, Patrinia villosa, Sarcandra glabra, Rhinacanthus nasutus and Glycyriza uralensis were chosen as candidates to study their characteristics. The OXOs from the above 5 plant species were all stable at high temperature and acid environment, and those from Echinacea purpurea, Rhinacanthus nasutus, and Patrinia villosa were not inhibited by NaCl and CaCl2 or hydrolysised by pepsin, indicating a high application potential. The enzyme from Glycyriza uralensis was not inhibited by NaCl and CaCl2 but sensitive to pepsin, while that from Sarcandra glabra was inhibited by NaCl and CaCl2 as well as pepsin. Related Articles | Metrics

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Extracting Technology of Total Flavone from Cyperus malaccensis var. brevifolius CHEN Hong-ming, HUANG Min-shen, FANG Yu-lin, ZENG Ya-mei, LIU Jian-qiu 2008, 37(02): 35-37.  Abstract ( )   PDF (852KB) ( )   Ultrasonic extraction and circular grain alcohol extraction technique were applied in the extraction on total flavone from Cyperus malaccensis var. brevifolius respectively. Both of them adopted the means of single factor experiment and L9(34) orthogonal test, analyzed with the soft ware SPSS11.5. The results were as follows. The optimum condition of ultrasonic extraction techniques was that the power of ultrasonic 400W, concentration of grain alcohol 60%, solid/liquid ratio 1:60, time of ultrasonic treatment 10min, the total flavone content 1.558%. The optimum condition of extraction with circumfluence of grain alcohol was that the temperature of water 90℃, concentration of grain alcohol 60%, solid/liquid ratio 1:60, circumfluence time 1.5h, total flavones content 2.011%. Comparing the two techniques, the content of total flavone extracted by circumfluence of grain alcohol was more than that extracted with ultrasonic, but ultrasonic extraction technique was excellent in saving time. Related Articles | Metrics

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Methods of Polyploid Induction of Cymbidium with Colchicine DENG Ying, ZHOU Ye, CHEN Ji-min 2008, 37(02): 38-40.  Abstract ( )   PDF (1718KB) ( )   The methods of polyploid induction of Cymbidium by colchicine were studied in this paper. The results showed that the methods of multiplicating PLBs gave a best result for polyploid induction, and about 16.7% protocorms developed into polyploidies. Cytological observation showed that the plantlets had the characteristics of polyploid phenotype and the chromosome number was 2n=80, but no mosaic phenomenon were observed, and the chromosome number of the control (no treated with colchicines) was 2n=40. Related Articles | Metrics

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Determination of Chelerythrine in Different Parts of Macleaya cordata by HPLC LI Xiao-meng, CHENG Li-hui, LIU Hui-qiong 2008, 37(02): 41-43.  Abstract ( )   PDF (882KB) ( )   The contents of the main constituent chelerythrine in different parts of Macleaya cordata were determined. HPLC method was adopted to measure the content of chelerythrine, Diamonsil C18 column was used with a mobile phase consisted of acetonitrile-0.2%phosphoric acid solution (22:78) by UV detector at 268nm。The results showed that fruits contained more chelerythrine than other parts of Macleaya cordata. It is scientific for use the fruit of Macleaya cordata as commercial herbs. Related Articles | Metrics

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Effects of Food Colors on Dyeing of Cut Flowers of Zantedeschia aethiopica HUANG Xiao-kun, HUANG Ni-ya, HUANG Xiao-dong, CHEN Qiu-feng 2008, 37(02): 44-45,49.  Abstract ( )   PDF (831KB) ( )   Through orthogonal experiment, the best condition of five food colors on dying fresh cut Zantedeschia aethiopica were studied. The results showed that the suitable dyeing density, time and pH for different plant pigment were Tartrazine(9g/L, 6h, 7.1), Ponceau 4R(12g/L, 5h, 8.5), Fruit Green (6g/L, 5.5h, 6.0), Sun Set Yellow(9g/L, 5h, 9.2), Amranth(9g/L or 12g/L, 6h, 9.4). The vase life became shorter after dyeing but still had 5~8 days for ornament. Related Articles | Metrics

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Residual Dynamics and Safe Usage Technologies of Indoxacarb in Cowpea YANG Shu-juan, GUO Jian-hui, Cai En-xing, Zhang Qi-hong 2008, 37(02): 46-49.  Abstract ( )   PDF (897KB) ( )   The residual degradation and safe usage technologies of 15% indoxacarb oil in cowpea were studied by gas chromatography analyzing. The results of determination showed that the LOD of this method was 0.01ng, LOQ was 0.005mg/kg; The average append recovery in cowpea ranged from 84.7%~92.8% and RSD ranged from 3.43%~5.27%. The results of residual degradation dynamics experiments showed that the original sediment of indoxacarb sprayed twice at 7d intervals in cowpea were more than that of once. The residual degradation dynamics of indoxacarb were followed first order kinetic equation. When it was sprayed once, the degradation dynamics equation was C=2.7180·e-0.2500t, DT50 was 2.8d and T0.99 was 18.4d. While it was twice, the degradation dynamics equation was C=2.3712·e-0.2404t, DT50 was 2.9d and T0.99 was 19.2d. When close to harvest, sprayed once with 48.21 g/hm2 of indoxacarb or twice at 7d intervals, the last residues in cowpea were less than 0.2mg/kg 11d after applied, the average residue was 0.103mg/kg and 0.111mg/kg respectively. The quality safety of products fulfils the MRL of Japan. Related Articles | Metrics

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Pharmacognostic identification of Uraria crinita LIN Mei-zhen 2008, 37(02): 50-52.  Abstract ( )   PDF (1108KB) ( )   Uraria crinita is a pharmaceutical plant usually used in traditional medicated diet. In this paper, the overall survey and distinguishes on morphology, pharmaceutical character, microscopic characteristics of Uraria crinita were described, and the physico-chemical identification was studied. Related Articles | Metrics

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Primary Studies on Flora of Indigenous Xylophyta for Traditional Chinese Medicine in Hainan Island SHAN Jia-lin, YU Lin, ZHENG Xue-qin 2008, 37(02): 53-56.  Abstract ( )   PDF (887KB) ( )   The flora composed of 332 species, belonging 206 genera and 70 families, characterized by tropical northern edge, was studied in the paper; in which the most species mainly distribute in the south-middle, the south-eastern and the southern region of Hainan island. Current situation and development in the future of the practical uses of medicinal plant resources were discussed here. Related Articles | Metrics

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Floristic Analysis of Daji Mountain Nature Reserve in Laizhou, Shandong Province WANG Jia-fu, WANG Sen-lin, Gao De-min 2008, 37(02): 57-60.  Abstract ( )   PDF (879KB) ( )   Daji Mountain Nature Reserve is located in Laizhou, Shandong Province. The flora possess the feature of richness in species, obvious dominant genera and families, many peculiar species, complex geographical compositions and distribution types, and ancient origin. It belongs to North China flora, having typical characteristics of temperate zone and some tropical affinity. Related Articles | Metrics

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A Study of Aquatic Medicinal Plants in Shandong Province BAI Wen-yong, GAO De-min, KANG Huai-xing 2008, 37(02): 61-63.  Abstract ( )   PDF (819KB) ( )   The aquatic medicinal plants in Shandong Province, about 128 species belonging to 82 genera in 65 families were described. These aquatic medicinal plants can be divided into 12 types according to their effectiveness. The strategies on protection and exploitation were also put forward. Related Articles | Metrics

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Evaluation in Introduction of Taiwan-related Landscape Plants in Xiamen HUANG Wei-lin 2008, 37(02): 64-68.  Abstract ( )   PDF (1825KB) ( )   This article looks back on the experiences of Xiamen Garden plants introduction recent 20 years and also the Taiwan businessmen's important role in this plants introduction. The investigation, summary and evaluation of seventy-seven kinds of garden plants introduced through ‘MinTai Chanel’ are described. This article also states and emphasis the guidance of management and scientific research of the ‘MinTai’ garden plants introduction. Related Articles | Metrics

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Tissue Cultrue and Rapid Propagation of Cymbidium ensifolium with Verge Line Pattern Leaves LI Li, LUO Jun-qin, NIE Zhen-peng, SUN Jian-hua, KE Fu-zhi, XU Jian-guo 2008, 37(02): 69-70.  Abstract ( )   PDF (2467KB) ( )   Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Potamogeton maackianus LI Hong-lin, LIU Feng, GAO Li, YANG Bo 2008, 37(02): 71-71.  Abstract ( )   PDF (694KB) ( )   Related Articles | Metrics

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Studies on Tissue Culture and Plant Regeneration of Anacardium occidentale LIN Feng, WANG Xiao-hua, LIANG Li-hong, ZHUANG Nan-sheng 2008, 37(02): 72-72.  Abstract ( )   PDF (810KB) ( )   Related Articles | Metrics

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Research Progress on Heterosis Utilization of Coconut WANG Ping, LIU Li-yun, TANG Long-xiang 2008, 37(02): 73-76.  Abstract ( )   PDF (889KB) ( )   The authors summarized the present situation of the utilization of coconut heterosis in China and abroad. Through the investigation of coconut hybrids on vegetative growth, yield, quality and resistance, it showed that coconut hybrids have strong hybrid vigor. Some prospects and comments of heterosis utilization were put forward. Related Articles | Metrics

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Advances on Antivirus and Antitumor Activities of Phyllanthus urinaria YUAN Yu-xi, LIN Yi, MING Yan-lin, TONG Qing-xuan 2008, 37(02): 77-80.  Abstract ( )   PDF (871KB) ( )   Many chemical constituents, such as alkaloids, flavonoids, 1ignans, terpenoids and tannins, has been identified from Phyllanthus urinaria. Extracts of Phyllanthus urinaria has been demonstrated to be active against virus and tumor, and be safe to animals tested. Phyllanthus urinaria is considered to be a promising Chinese medicine from which natural drugs would be developed. In this paper, the advances on the chemical constituents, antivirus and antitumor activities of Phyllanthus urinaria are presented. Related Articles | Metrics