植物细胞悬浮培养在次级代谢产物生产中的应用

doi:10.3969/j.issn.1009-7791.2025.03.013

亚热带植物科学 ›› 2025, Vol. 54 ›› Issue (3): 346-355.DOI: 10.3969/j.issn.1009-7791.2025.03.013

植物细胞悬浮培养在次级代谢产物生产中的应用

李惠华*，陈菲，王伟，叶秋萍，吴美芳，施建羽

(福建省亚热带植物研究所 / 福建省亚热带植物生理生化重点实验室，福建厦门 361006)

收稿日期:2024-11-11 接受日期:2025-01-14 出版日期:2025-06-30 发布日期:2025-09-08
通讯作者: 李惠华
基金资助:
厦门市自然科学基金面上项目(3502Z20227259)；三明市科技计划项目(2023-N-19)

Application of Plant Cell Suspension Culture in the Production of Secondary Metabolites

LI Hui-hua*, CHEN Fei, WANG Wei, YE Qiu-ping, WU Mei-fang, SHI Jian-yu

(Fujian Institute of Subtropical Botany / Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Xiamen 361006, Fujian China)

Received:2024-11-11 Accepted:2025-01-14 Online:2025-06-30 Published:2025-09-08
Contact: LI Hui-hua

摘要/Abstract

摘要： 植物细胞悬浮培养已逐渐成为替代传统方法用于大规模生产植物衍生代谢物的生物技术。本文综述植物悬浮细胞培养的技术优势及与新热点外泌体结合的研究进展，从愈伤组织培养到产品形成的技术流程，优良愈伤组织诱导阶段外植体的基因型、外植体取材部位、激素组合筛选，液体培养阶段培养基优化、诱导子添加，生物发酵罐阶段的简要历程和重要影响因素等。同时阐述商品化的细胞系及产物的特征和我国植物悬浮培养技术研究存在问题及原因。

关键词: 植物细胞, 悬浮细胞, 悬浮培养, 次生代谢产物, 研究进展

Abstract: Plant cell culture has gradually become a biotechnology that replaces traditional methods for the large-scale production of plant-derived metabolites. This article reviews the technical advantages of plant suspension cell culture, the integration points with the new research hotspot of exosomes; the technical process from callus culture to product formation; the key points of genotype of explants, the parts of explants, and hormone combinations during the induction phase of good callus tissue; the key points of medium optimization and inducer addition during the liquid culture phase; and a brief course and important influencing factors during the biofermentation tank phase. At the same time, it elaborates on the characteristics of commercialized cell lines and products, as well as the current status and reasons for plant suspension culture technology research in our country.

Key words: plant cells, suspension cells, suspension culture, secondary metabolites, research advance

中图分类号:

Q946

李惠华, 陈菲, 王伟, 叶秋萍, 吴美芳, 施建羽. 植物细胞悬浮培养在次级代谢产物生产中的应用[J]. 亚热带植物科学, 2025, 54(3): 346-355.

LI Hui-hua, CHEN Fei, WANG Wei, YE Qiu-ping, WU Mei-fang, SHI Jian-yu. Application of Plant Cell Suspension Culture in the Production of Secondary Metabolites[J]. Subtropical Plant Science, 2025, 54(3): 346-355.

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植物细胞悬浮培养在次级代谢产物生产中的应用

Application of Plant Cell Suspension Culture in the Production of Secondary Metabolites

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