巨桉Dof基因家族鉴定及表达分析

doi:10.3969/j.issn.1009-7791.2026.01.001

亚热带植物科学 ›› 2026, Vol. 55 ›› Issue (1): 1-11.DOI: 10.3969/j.issn.1009-7791.2026.01.001

• 研究论文 • 下一篇

巨桉Dof基因家族鉴定及表达分析

杨玉婷，冯子怡，吴蔼民^*

(华南农业大学林学与风景园林学院 / 广东省森林植物种质创新与利用重点实验室，广东广州 510642)

收稿日期:2025-09-20 接受日期:2025-12-26 出版日期:2026-02-28 发布日期:2026-03-01
通讯作者: 吴蔼民
基金资助:
科技部科技创新2030-重大项目(2023ZD0405702-03)

Identification and Expression Analysis of the Dof Gene Family in Eucalyptus grandis

YANG Yu-ting, FENG Zi-yi, WU Ai-min^*

(College of Forestry and Landscape Architecture, South China Agricultural University / Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, Guangdong China)

Received:2025-09-20 Accepted:2025-12-26 Online:2026-02-28 Published:2026-03-01
Contact: WU Ai-min

摘要/Abstract

摘要： Dof (DNA binding with one finger)家族作为植物中特有的转录因子，在植物生长发育、光形态建成以及逆境响应等生物学过程中具有重要作用。本研究基于巨桉Eucalyptus grandis Dof基因家族，系统分析了其家族成员、进化关系、基因结构、顺式作用元件及共线性分析。结果显示，在巨桉的基因组中鉴定出26个Dof基因家族成员，其蛋白质长度在183到516个氨基酸之间，等电点普遍大于7.0，且多数定位于细胞核。染色体定位显示26个EgrDofs基因不均匀分布在10条染色体上，且所有蛋白均含典型Dof锌指结构域。启动子顺式作用元件分析揭示，EgrDofs基因广泛参与光感应、激素响应和非生物胁迫调控。EgrDofs在不同生长时间段的叶片表达量较低，在茎和韧皮部中表达量较高。该研究可为Dof基因的生物学功能和调控网络提供参考基础。

关键词: 巨桉, Dof转录因子, 生物信息学, 表达分析

Abstract: The Dof (DNA binding with one finger) family of transcription factors is a special kind found in plants and is crucial for biological processes including photomorphogenesis, stress response, and plant growth and development. This research focuses on the Eucalyptus grandis Dof gene family, systematically analyzing its evolutionary relationships, gene structure, cis-acting elements, member identification, and synteny analysis. According to the findings, 26 members of the Dof gene family were found in the E. grandis genome. These members generally reside in the nucleus, with protein lengths ranging from 183 to 516 amino acids, and an isoelectric point that is often higher than 7.0. According to chromosomal location, all proteins have the characteristic Dof zinc finger domain, and the 26 EgrDof genes are widely dispersed over 10 chromosomes. According to an analysis of the cis-acting regions in the promoters, abiotic stress regulation, hormone response, and light sensing are all extensively impacted by genes. EgrDofs expression levels are higher in stems and phloem, while levels are comparatively low in leaves at various development stages. This study offers a strong basis for comprehending the regulatory networks and biological functions of Dof genes.

Key words: Eucalyptus grandis, Dof transcription factor; bioinformatics, tissue expression

中图分类号:

Q943.2

杨玉婷, 冯子怡, 吴蔼民. 巨桉Dof基因家族鉴定及表达分析[J]. 亚热带植物科学, 2026, 55(1): 1-11.

YANG Yu-ting, FENG Zi-yi, WU Ai-min. Identification and Expression Analysis of the Dof Gene Family in Eucalyptus grandis[J]. Subtropical Plant Science, 2026, 55(1): 1-11.

参考文献

[1] Yanagisawa S. The Dof family of plant transcription factors [J]. Trends In Plant Science, 2002, 7(12): 555–560.

[2] Shigyo M, Tabei N, Yoneyama T, Yanagisawa S. Evolutionary processes during the formation of the plant-specific Dof transcription factor family [J]. Plant And Cell Physiology, 2007, 48(1): 179–185.

[3] Guo Y, Qiu L J. Genome-wide analysis of the Dof transcription factor gene family reveals soybean-specific duplicable and functional characteristics [J]. PLoS One, 2013, 8(9): e76809.

[4] Jiang Y, Zeng B, Zhao H, Zhang M, Xie S, Lai J. Genome-wide transcription factor gene prediction and their expressional tissue-specificities in maize [J]. Journal of Integrative Plant Biology, 2012, 54(9): 616–630.

[5] Cai X, Zhang Y, Zhang C, Zhang T, Hu T, Ye J, Zhang J, Wang T, Li H, Ye Z. Genome-wide analysis of plant-specific Dof transcription factor family in tomato [J]. Journal of Integrative Plant Biology, 2013, 55(6): 552–566.

[6] Wen C L, Cheng Q, Zhao L, Mao A, Yang J, Yu S, Weng Y, Xu Y. Identification and characterisation of Dof transcription factors in the cucumber genome [J]. Scientific Reports, 2016, 6: 23072.

[7] Zhou K, Zhang Y, Zhou Y, Xu M, Yu S. Production of gypenoside XVII from ginsenoside Rb1 by enzymatic transformation and their anti–inflammatory activity in vitro and in vivo [J]. Molecules, 2023, 28(19). DOI: 10.3390/molecules28197001.

[8] Rueda-Romero P, Barrero-Sicilia C, Gomez-Cadenas A, Carbonero P, Onate-Sanchez L. Arabidopsis thaliana Dof6 negatively affects germination in non-after-ripenedseeds and interacts with TCP14 [J]. Journal of Experimental Botany, 2012, 63(5): 1937–1949.

[9] Kim H S, Kim S J, Abbasi N, Bressan R A, Yun D J, Yoo S D, Kwon S Y, Choi S B. The Dof transcription factor Dof 5.1 influences leaf axial patterning by promoting revoluta transcription in Arabidopsis [J]. Plant Journal, 2010, 64(3): 524–535.

[10] Isabel-LaMoneda I, Diaz I, Martinez M, Mena M, Carbonero P. SAD: a new Dof protein from barley that activates transcription of a cathepsin B-like thiol protease gene in the aleurone of germinating seeds [J]. Plant Journal, 2003, 33(2): 329–340.

[11] Zhang B, Chen W, Foley R C, Buttner M, Singh K B. Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences [J]. Plant Cell, 1995, 7(12): 2241–2252.

[12] Krohn N M, Yanagisawa S, Grasser K D. Specificity of the stimulatory interaction between chromosomal HMGB proteins and the transcription factor Dof2 and its negative regulation by protein kinaseCK2-mediated phosphorylation [J]. Journal of Biological Chemistry, 2002, 277(36): 32438–32444.

[13] Vicente-Carbajosa J, Moose S P, Parsons R L, Schmidt R J. A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2 [J]. Proceedings of The National Academy of Sciences of The United States of America, 1997, 94(14): 7685–7690.

[14] Diaz I, Vicente-Carbajosa J, Abraham Z, Martinez M, Isabel-La M I, Carbonero P. The GAMYB protein from barley interacts with the Dof transcription factor BPBF and activates endosperm- specific genes during seed development [J]. Plant Journal, 2002, 29(4): 453–464.

[15] Renau-Morata B, Carrillo L, Dominguez-Figueroa J, Vicente-Carbajosa J, Molina R V, Nebauer S G, Medina J. CDF transcription factors: plant regulators to deal with extreme environmental conditions [J]. Journal of Experimental Botany, 2020, 71(13): 3803–3815.

[16] Khaksar G, Sangchay W, Pinsorn P, Sangpong L, Sirikantaramas S. Genome-wide analysis of the Dof gene family in durian reveals fruit ripening-associated and cultivar-dependent Dof transcription factors [J]. Scientific Reports, 2019, 9(1): 12109.

[17] 林怡, 周江. 番木瓜Dof基因家族的全基因组鉴定及其在不同光处理下的表达分析[J]. 中国南方果树, 2024, 53(6): 57–67.

[18] Wasserman W W, Sandelin A. Applied bioinformatics for the identification of regulatory elements [J]. Nature Reviews Genetics, 2004, 5(4): 276–287.

[19] Chen P, Yan M, Li L, He J, Zhou S, Li Z, Niu C, Bao C, Zhi F, Ma F, Guan Q. The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance [J]. Horticulture Research, 2020, 7(1): 195.

[20] Zhou Y, Cheng Y, Wan C, Li J, Yang Y, Chen J. Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon [J]. Peer J, 2020, 8: e8358.

[21] Wang Z, Wong D, Chen Z, Bai W, Si H, Jin X. Emerging roles of plant DNA-binding with one finger transcription factors in various hormone and stress signaling pathways [J]. Frontiers in Plant Science, 2022, 13: 844201.

[22] Cao L, Ye F, Fahim AM, Ma C, Pang Y, Zhang X, Zhang Q, Lu X. Transcription factor ZmDof22 enhances drought tolerance by regulating stomatal movement and antioxidant enzymes activities in maize (Zea mays L.) [J]. theoretical and Applied Genetics, 2024, 137(6): 132.

[23] 王杰, 李坤, 方能干, 刘秉兴, 杨梅. 不同年龄序列巨尾桉人工林生长的坡位效应[J]. 安徽农业科学, 2023, 51(8): 142–144.

[24] Fan C, Lyu M, Zeng B, He Q, Wang X, Lu M Z, Liu B, Liu J, Esteban E, Pasha A, Provart N J, Wang H, Zhang J. Profiling of the gene expression and alternative splicing landscapes of Eucalyptus grandis [J]. Plant Cell and Environment, 2024, 47(4): 1363–1378.

[25] Kapli P, Yang Z, Telford M J. Phylogenetic tree building in the genomic age [J]. Nature Reviews Genetics, 2020, 21(7): 428–444.

[26] 黄巧玉, 杨珺菲, 汪旭祥, 陆晓莹, 李晓谊, 李永强, 徐丽珊, 宗宇, 郭卫东. 蓝莓B-box型锌指蛋白基因家族的鉴定及其在果实发育期的表达模式[J]. 浙江师范大学学报(自然科学版), 2024, 47(2): 185–195.

[27] 刘楚楚, 吴玉香. 棉花RCI2基因家族的鉴定及表达分析[J]. 山西农业大学学报(自然科学版), 2021, 41(4): 41–49.

[28] 秦晨, 解雪华, 李路, 李俊, 米要磊, 曹惠妍, 孙伟, 马伟, 万会花, 刘秀波. 七叶树Dof转录因子家族鉴定及表达分析[J]. 中国中药杂志, 2024, 49(22): 6082–6092.

[29] Lijavetzky D, Carbonero P, Vicente-Carbajosa J. Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families [J]. BMC Evolutionary Biology, 2003, 3: 17.

[30] Yang X, Tuskan G A, Cheng M Z. Divergence of the Dof gene families in poplar, Arabidopsis, and rice suggests multiple modes of gene evolution after duplication [J]. Plant Physiology, 2006, 142(3): 820–830.

[31] Ramachandran V, Tobimatsu Y, Masaomi Y, Sano R, Umezawa T, Demura T, Ohtani M. Plant-specific Dof transcription factors Vascular-related Dof1 and Vascular-related Dof2 regulate vascular cell differentiation and lignin biosynthesis in Arabidopsis [J]. Plant Molecular Biology, 2020, 104(3): 263–281.

编辑推荐

Metrics

闽ICP备11008787号-5　

巨桉Dof基因家族鉴定及表达分析

Identification and Expression Analysis of the Dof Gene Family in Eucalyptus grandis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

[1]	凌腾泓, 黄稚清, 谢进聪, 吴蔼民. 巨桉HDAC基因家族的全基因组鉴定与分析[J]. 亚热带植物科学, 2025, 54(4): 365-377.
[2]	王安邦，叶兴状，杨巧莹，刘美利，陈志云，张国防. 蕈树TCP基因家族鉴定及组织表达分析[J]. 亚热带植物科学, 2025, 54(3): 249-260.
[3]	赵金涛, 叶兴状, 王安邦, 陈志云, 翁慧莹, 张国防. 枫香树TCP基因家族鉴定与表达分析[J]. 亚热带植物科学, 2025, 54(2): 109-120.
[4]	王安邦, 叶兴状, 赵金涛, 陈志云, 翁慧莹, 林茂, 张国防. 蕈树KNOX基因家族鉴定及组织表达分析[J]. 亚热带植物科学, 2025, 54(1): 1-10.
[5]	罗岸，左紫怡，焦雄，刘夏. 烟草合子时期表达基因NtZE1的克隆及结构分析[J]. 亚热带植物科学, 2019, 48(02): 103-108.
[6]	王会全，李亮，余志雄，袁亚芳，吴少华. PP2C相关基因在砂梨休眠进程中的表达分析[J]. 亚热带植物科学, 2018, 47(04): 305-311.
[7]	刘薇,吴晶晶,陈宏文. 米曲霉6-磷酸葡萄糖脱氢酶基因gsdA的克隆及生物信息学分析[J]. 亚热带植物科学, 2012, 41(03): 11-15.
[8]	韦大器,时群. 尾巨桉胚培养与快速繁殖研究[J]. 亚热带植物科学, 2008, 37(04): 56-56,58.
[9]	郭祥泉,许少洪,郑经池,林方良,许接眉,刘进山. 福建省中部地区抗寒速生尾巨桉优株筛选[J]. 亚热带植物科学, 2004, 33(04): 45-47,51.
[10]	邹小兴,梁一池,孙小霞. 巨桉家系主要产量性状遗传相关分析[J]. 亚热带植物科学, 2003, 32(03): 21-24.