胡萝卜四分体时期的花药在药室内壁和绒毡层细胞中积累淀粉粒,随着花药的发育,花粉先出现大液泡,同时药室内壁和绒毡层细胞中淀粉粒消失;以后花粉中的大液泡消失,在花粉细胞质中出现淀粉粒。伴随着花粉的发育,绒毡层细胞退化,在细胞中积累较多的脂类物质,同时花粉中脂类物质含量也明显增加。胡萝卜成熟花粉粒的储存物主要为脂滴,也有少部分淀粉颗粒。胡萝卜花药在特定时间和特定部位积累营养储存物的过程也是其发育的一个特征。
With pollen development, a big vacuole appeared in the pollen and the starch grains in endothecium and tapetum disappeared. Then the big vacuole decomposed and some starch grains appeared in pollen. Tapetal cells degenerated with pollen development. Some lipid material accumulated in tapetal cells and the lipid material in pollen grains also increased. The most store material in mature pollen is lipid and small part of starches. The process of nutrition material accumulated in pollen with special time and part is also a developmental feature.
[1] 胡适宜. 被子植物生殖生物学[M]. 北京: 高等教育出版社, 2005: 29-92.
[2] Rudramuniyapa C K, Annigeri B G. Histochemical observations on the sporogenous tissue and tapetum in the anther of Euphorbia[J]. Cytologia, 1985,50: 39-48.
[3] Tiwari S C, Gunning B E S. An ultrastructural cytochemical and immunofluorescence study of postmeiotic development of plasmodial tapetum in Tradescantia virginiana L. and its relevance to the pathway of sporopollenin secretion[J]. Protoplasma, 1986,133: 100-144.
[4] Calzoni G L, Speranza A, Caramiello R, et al. Wall ultrastructure and biochemical features of the Juglaus rega L. and Juglaus nigra L. male gametophyte[J]. Sexual Plant Reproduction, 1990,(3): 139-146.
[5] 田惠桥,朱学艺. 被子植物生殖生物学[M]. 北京: 科学出版社, 2012: 1-15.
[6] 胡适宜,徐丽云. 显示环氧树脂厚切片中多糖、蛋白质和脂类的细胞化学方法[J]. 植物学报, 1990,32(11): 841-846.
[7] 罗玉英,胡适宜. 玉竹生殖细胞壁在发育中的变化[J]. 植物学报, 1995,37(1): 7-13.
[8] 谢潮添,杨延红,朱学艺,等. 白菜细胞核雄性不育花药的细胞化学观察[J]. 实验生物学报, 2004,37: 295-302.
[9] 徐青,王仙琴,田惠桥,等. 枸杞花药发育过程中脂滴和淀粉粒的分布特征[J]. 分子细胞生物学报, 2006,39(2): 103-110.
[10] 魏冬梅,菅明霞,邓桦,等. 洋葱花药发育过程中的细胞化学研究[J]. 分子细胞生物学报, 2007,40(6): 451-457.
[11] 郑松,魏冬梅,林美珍,等. 巴戟天花药发育过程中多糖和脂滴分布特征[J]. 植物生理学通讯, 2010,46(3): 231-235.