应用正交设计法优选黄独脱毒苗快繁培养基

尹明华; 洪森荣; 徐卫红; 王艾平

doi:10.3969/j.issn.1009-7791.2009.03.006

亚热带植物科学 >

2009 , Vol. 38 >Issue 03: 24 - 26

DOI: https://doi.org/10.3969/j.issn.1009-7791.2009.03.006

研究报告

应用正交设计法优选黄独脱毒苗快繁培养基

尹明华 ,
洪森荣 ,
徐卫红 ,
王艾平

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上饶师范学院生命科学系, 江西上饶 334001

尹明华(1973-),女,江西吉安人,硕士,讲师,从事生物技术研究。

收稿日期: 2009-02-11

网络出版日期: 2009-09-10

基金资助

江西省教育厅科技项目(GJJ09374);上饶师范学院院级科技项目(SR0911)

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Study on Rapid Propagation Medium for Virus-free Plantlets of Dioscorea bulbifera by Orthogonal Design

YIN Ming-hua ,
HONG Sen-rong ,
XU Wei-hong ,
WANG Ai-ping

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Department of Life Science, Shangrao Normal University, Shangrao 334001, Jiangxi China

Received date: 2009-02-11

Online published: 2009-09-10

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摘要

通过正交设计法研究黄独脱毒苗的快繁培养基,考察KT、NAA和2,4-D对黄独脱毒苗快繁的影响。结果表明,黄独脱毒苗快繁的最佳培养基为MS+KT 2.0 mg/L+NAA 0.5 mg/L。

关键词： 黄独; 脱毒苗; 快繁培养基; 优选

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尹明华 , 洪森荣 , 徐卫红 , 王艾平 . 应用正交设计法优选黄独脱毒苗快繁培养基[J]. 亚热带植物科学, 2009 , 38(03) : 24 -26 . DOI: 10.3969/j.issn.1009-7791.2009.03.006

Abstract

The rapid propagation medium for virus-free plantlets of Dioscorea bulbifera were studied by orthogonal design,and the effects of rapid propagation medium including different concentrations of KT,NAA and 2,4-D on rapid propagation of Dioscorea bulbifera were also investigated.The results showed that the best rapid propagation medium for virus-free plantlets of Dioscorea bulbifera was MS+KT 2.0 mg/L+NAA 0.5 mg/L.

Key words： Dioscorea bulbifera; virus-free plantlets; rapid propagation medium; optimization

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