以未成熟水稻‘博Ⅱ优15’种子为材料,用不同浓度GA₃、ABA浸种24 h,研究其对水稻种子萌发的影响。结果表明,GA₃ 0.10~2.00 g/L处理能极显著促进种子萌发,其中0.50 g/L效果最好,第4天发芽率高于对照(CK)56%;浓度大于0.50 g/L ABA处理显著抑制种子萌发;GA₃与ABA协同处理时,GA₃/ABA=1:1(浓度0.05 g/L)和GA₃/ABA=1:2(浓度0.01 g/L)均能极显著促进种子萌发,第7天种子发芽率分别比CK高30%和20%。进一步研究表明,GA₃和ABA对未成熟水稻种子萌发的影响与α-淀粉酶活性相关。

Influence of GA₃ and ABA on germination of immature rice Bo II You 15 seeds were investigated.The results of treatments with different concentration of GA₃ and/or ABA and distilled water (as control,CK) were as follows.Among those treatments,GA₃ with 500 mg/L was the best to promote germination,and the germination rate could break through to 80% on the fourth day,which was 56% higher than that of CK.Treatment of ABA with more than 500 mg/L,could inhibit the germination markedly.When treated with both GA₃ and ABA,with the ratio of GA₃/ABA=1(50 mg/L),GA₃/ABA=1/2(10 mg/L),promoted the germination respectively,and made the germination rate get to 60% and 50% on the seventh day,which was 30% and 20% higher than that of CK.Further study indicated that such regulation of GA₃ and ABA on germination of immature rice seeds was closely related to the α-amylase activity.