研究报告

‘巴斗杏’组培快繁体系建立与耐盐植株筛选

  • 杜雪玲 ,
  • 余如刚 ,
  • 宋运贤 ,
  • 张振霞
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  • (1.淮北师范大学 生命科学学院 资源植物学安徽省重点实验室,安徽 淮北235000;2.韩山师范学院 生物系,广东 潮州 521041)
杜雪玲(1976-),女,河南扶沟人,硕士,讲师,从事植物细胞工程研究。

收稿日期: 2010-12-27

  修回日期: 2010-12-27

  网络出版日期: 2011-06-10

基金资助

安徽省高校自然科学研究项目(KJ2008B230)

Establishment of Tissue Culture and Rapid Propagation System of Armeniaca vulgaris ‘Badou’and Selection of Salt-tolerant Plant

  • DU Xue-Ling ,
  • YU Ru-Gang ,
  • SONG Yun-Xian ,
  • ZHANG Zhen-Xia
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  • (1.Anhui Key Laboratory of Plant Resources and Biology, College of Life Science, Huaibei Normal University, Huaibei 235000, Anhui China; 2.Department of Biology, Hanshan Normal University, Chaozhou 521041, Guangdong China)

Received date: 2010-12-27

  Revised date: 2010-12-27

  Online published: 2011-06-10

摘要

以淮北黄里‘巴斗杏’茎段为外植体,MS为基本培养基,通过茎段诱导植株再生及进行耐盐筛选。结果表明:采用4、5月‘巴斗杏’茎段用0.1%升汞灭菌8 min较适宜;在含有1.0 mg/L 6-BA和0.1 mg/L IBA的MS培养基上茎段增殖较快,生长旺盛;较理想的生根培养基为MS + NAA 0.1 mg/L + IBA 0.2 mg/L,生根率达46.3%;‘巴斗杏’组培苗进行耐盐筛选的适宜盐浓度为0.4%~0.8%,筛选植株与对照相比差异显著。

本文引用格式

杜雪玲 , 余如刚 , 宋运贤 , 张振霞 . ‘巴斗杏’组培快繁体系建立与耐盐植株筛选[J]. 亚热带植物科学, 2011 , 40(02) : 34 -38 . DOI: 10.3969/j.issn.1009-7791.2011.02.011

Abstract

The stem of Armeniaca vulgaris ‘Badou’ was used as explants and MS as basic substrate to induce plant regeneration and select salt-tolerant plant. The results showed that the stem of ‘Badou’ in April and May sterilized by 0.1% mercury for 8min was suitable, the stem in MS culture medium with 1.0mg/L 6-BA and 0.1 mg/L IBA proliferated quickly and grew rushingly, the perfect rooting medium was MS+0.1 mg/L NAA + 0.2 mg/L IBA, with its rooting rate of 46.3%, and the optimum salt concentration in selection of salt-tolerant tissue culture seedlings was 0.4~0.8, there were also significant difference between plants selected and their contrast.

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