采用ISSR分子标记鉴定方法及HPLC色谱方法对武夷山及周边地区5份黄精植物样本进行DNA分子标记鉴定及指纹图谱分析，并通过聚类分析软件分别探讨其遗传相关聚类图谱。ISSR分子标记法能较好地对5份黄精植物样本进行区分，HPLC指纹图谱分析同样表明5份黄精样本化学成分存在一定的差别。DNA分子标记及HPLC指纹图谱分析的遗传相关聚类分析结果相似，表明武夷山及周边地区的野生黄精种质资源存在明显的差别。
The purpose of this project was to identify and analyze the genetic relationships of 5 kinds of Polygonatum in Wuyishan and its surrounding areas. Using ISSR marker and HPLC molecular identification method for chromatographic methods analysis of DNA markers for identification and fingerprinting of 5 Polygonatum samples, the genetic correlation map of Polygonatum was studied by cluster analysis software. ISSR molecular marker method could be used to distinguish Polygonatum of Wuyishan and its surrounding areas. HPLC fingerprint analysis also showed that there were some differences in the composition of Wuyishan and its surrounding distribution. The results of genetic correlation cluster analysis were similar by ISSR and HPLC. There were obvious differences in the germplasm resources of Wuyishan and its surrounding areas from the DNA molecular markers and HPLC fingerprinting analysis results.
[1] 中国药典委员会主编. 黄精,中国药典(一部)[M]. 北京: 中国医药科技出版社, 2015: 307.
[2] Tamura M N, Schwarzbach A E, Kruse S, Reski R. Biosystemic studies on the genus Ploygonatum(Convallariaceae)IV. molecular phylogenetic analysis based on the restriction site mapping of the chloroplast gene trnK[J]. Feddes Repertorium, 1997,108: 159—168.
[3] 吴世安,吕海亮,杨继,饶广远,尤瑞麟,葛颂,钟扬. 叶绿体DNA片段的RFLP分析在黄精族系统学研究中的应用[J]. 植物分类学报, 2000,38(2): 97—110.
[4] 周晔,王润玲,唐铖,安适之,姚智. ISSR法鉴定中药黄精与卷叶黄精[J]. 天津医科大学学报, 2006,12(2): 178—180.
[5] 何正文,刘运生,陈立华,曹美鸿,夏家辉. 正交设计直观分析法优化条件(PCR)[J]. 湖南医科大学学报, 1998,23(4): 403—404.
[6] 李房英,黄彦晶,吴少华. 三角梅ISSR体系的建立与优化[J]. 生物技术通报, 2010(7): 142—145.
[7] 周守标,张小平. 安徽省黄精属(Polygonatum)植物的分支分析[J]. 广西植物, 2000,20(4): 329—331.
[8] 林萍,张含国,谢运海. 正交设计落叶松ISSR-PCR反应体系[J]. 生物技术, 2005,15(5): 34—36.
[9] 胡甦,王永清,陶炼. 三角紫叶酢浆草ISSR体系的建立与优化[J]. 草业学报, 2011,20(5): 142—150.
[10] Monika S, Jakub S, Kornelia P. Comparison of three Polygonatum species from Poland based on DNA markers[J]. Annales Botanici Fennici, 2006,43: 379—388.
[11] 张红梅,邵元华,龙甜甜,项艳,王进,汤锋. 多花黄精ISSR反应体系的建立及正交优化设计[J]. 安徽农业大学学报, 2012,39(3): 412—416.
[12] 赵欣,刘晓蕾,兰晓继,刘峰,梁宗锁. 黄精的高效液相色谱指纹图谱[J]. 西北农业学报, 2011,20(2): 114—119.
[13] 焦劼,陈黎明,孙瑞泽,刘峰,马存德,梁宗锁. 不同产地黄精主要化学成分比较及主成分分析[J]. 中药材, 2016,39(3): 519—522.
[14] 候小平,何新新,苏薇薇. 中药指纹图谱质量控制技术[J]. 中药材, 2001,24(5): 370.
[15] 张文生,叶正良,岳洪水,宋晓涛. 丹参药材指纹图谱研究[J]. 中药材, 2001,24(7): 478.
闽公网安备 35020602000789号