以叶下珠Phyllanthus urinaria带节茎段为外植体，研究生长调节剂浓度、培养基对茎段诱导芽的影响，再以叶下珠无菌芽为材料，开展丛生芽增殖培养、生根培养和炼苗移栽技术研究。结果表明，适合叶下珠茎段诱导芽的培养基为1/2MS + 6-BA 2.0 mg·L-1 + IBA 0.2 mg·L-1 + 琼脂7.5 g·L-1 + 蔗糖30 g·L-1 + 活性炭0.5 g·L-1 (pH 5.8～6.0)，诱导率达100%；适合叶下珠芽增殖的培养基为1/2MS + 6-BA 1.0 mg·L-1 + IBA 0.2 mg·L-1 + 琼脂7.5 g·L-1 + 蔗糖30 g·L-1 (pH 5.8～6.0)，平均增殖倍数为3.8；生根适宜培养基为1/2MS + IBA 0.5 mg·L-1 + 琼脂7.5 g·L-1 +蔗糖20 g·L-1 (pH 5.8～6.0)，生根率达100%；最佳炼苗时间为闭盖2 d后开盖3 d，移栽成活率86.6%。
Using stem-segment with node of Phyllanthus urinaria as explants, effects of different culture medium and hormones on induction of P. urinaria were analyzed. Using germ-free shoots as material, proliferation, rooting, acclimatization and transplant of P. urinaria were studied. The results showed that the combination of 1/2MS + 6-BA 2.0 mg·L-1 + IBA 0.2 mg·L-1 + agar 7.5 g·L-1 + sucrose 30 g·L-1 + activated carbon 0.5 g·L-1 (pH 5.8—6.0) was the optimal medium for buds induction with induction rate of 100%; the optimum for multiple shoots was the combination of 1/2MS + 6-BA 1.0 mg·L-1 + IBA 0.2 mg·L-1 + agar 7.5 g·L-1 + sucrose 30 g·L-1 (pH 5.8—6.0) and the average multiplication coefficient was 3.8; It was best to rooting under the condition of 1/2MS + IBA 0.5 mg·L-1 + agar 7.5 g·L-1 + sucrose 20 g·L-1 (pH 5.8—6.0) and the rooting rate was 100%. After closing cap 2 d and then opened cap 3 d to practice seedlings and transplant, surviving of tube plantlet rates could reach 86.6%.
[1] 胡显镜,梁艳,于荣敏. 叶下珠愈伤组织快速培养体系研究[J]. 中药材, 2011,33(3): 334—336.
[2] 李勉民. 常见药草图说[M]. 香港: 读者文摘远东有限公司, 2002.
[3] 梁定仁,蒙爱东,孙西. 广西叶下珠属药用植物[J]. 中国民族医药杂志, 2000,12(6): 49.
[4] 仲英,左春旭,李风琴,丁杏苞,姚庆强,吴克霞,张琴冈,王志玉,周玲,王菊,兰静,王晓静. 叶下珠化学成分及其抗乙型肝炎病毒活性的研究[J]. 中国药学杂志, 1998,23(6): 363.
[5] 戴卫波,肖文娟. 叶下珠药理作用研究进展[J]. 药物评价研究, 2016,39(3): 498—500.
[6] 程艳刚,裴妙荣,孔祥鹏,李慧峰,刘艳,谭金燕. 叶下珠化学成分和药理作用研究进展[J]. 辽宁中医药大学学报, 2016,18(4): 238—242.
[7] 戴卫波,吴凤荣,肖文娟,陈少娜. 叶下珠甲醇提取物抗炎镇痛及体外抑菌作用研究[J]. 中华中医药学刊, 2016,34(4): 978—981.
[8] 吴莹,雷宇,王媛媛,李姝玉,禄颖,王旭丹. 叶下珠提取物对急性乙型肝炎小鼠乙型肝炎病毒复制及其抗原表达的影响[J]. 中国中医药信息杂志, 2014,21(12): 51—54.
[9] Wu Y, Lu Y, Li S Y, Song Y H, Hao Y, Wang Q. Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro[J]. Chinese Journal of Integrative Medicine, 2015,21(12): 938—943.
[10] Jung J, Kim N K, Park S, Shin H J, Hwang S G, Kim K. Inhibitory effect of Phyllanthus urinaria L. extract on the replication of lamivudine-resistant hepatitis B virus in vitro[J]. BMC Complementary and Alternative Medicine, 2015,15(1): 255.
[11] Tong G D, Zhang X, Zhou D Q, Wei C S, He J S, Xiao C L, Liu X L, Zheng Y J, Chen S N, Tang H H. Efficacy of early treatment on 52 patients with preneoplastic hepatitis B virus-associated hepatocellular carcinoma by compound Phyllanthus urinaria L.[J]. Chinese Journal of Integrative Medicine, 2014,20(4): 263—271.
[12] 常莉,薛建平. 叶下珠愈伤组织的诱导与培养[J]. 中草药, 2008,39(11): 1723—1726.
[13] 韩晓玲,秋小冬,王冰雪,杜勇军,贾敬芬. 叶下珠茎节组织培养与快速繁殖[J]. 植物生理学通讯, 2006,42(4): 679.
[14] 张树峰,明艳林,林毅,陈良华,童庆宣. 叶下珠属植物活性成分柯里拉京研究进展[J]. 亚热带植物科学, 2010,39(4): 79—83.
闽公网安备 35020602000789号