在雷公藤Tripterygium wilfordii组织培养生根培养阶段以柠檬酸、丙酮酸钠、乙酸钠、L-酪氨酸、L-苯丙氨酸、赖氨酸为前体物质，添加到培养基中，培养30 d后，观测外植体生根情况并测定甲素含量。结果表明，丙酮酸钠3.0 mg·L-1在光照条件及乙酸钠1.0 mg·L-1在黑暗条件培养中可获得最大的不定根量，赖氨酸1.0 mg·L-1在光照条件及L-酪氨酸1.0 mg·L-1在黑暗条件中培养可获得较高含量甲素。
With citric acid, sodium pyruvate, sodium acetate, L-tyrosine, L-phenylalanine, lysine as precursors in tissue culture of Tripterygium wilfordii, the rooting of explants and the content of triptolide was determined after 30 d. The results showed that 3.0 mg稬-1 pyruvic acid in illumination condition and 1.0 mg稬-1 sodium acetate in dark condition could get the maximum amount of adventitious roots, while 1.0 mg稬-1 lysine in illumination condition and 1.0 mg稬-1 tyrosine in dark condition could get the highest concentration of triptolide.
[1] 尹作鸿,朱蔚华,于德全. 雷公藤组织培养物中化学成分的研究[J]. 天然产物研究与开发, 1991,3(2): 46—51.
[2] 王丹,王振月,王宗权,陈金铭. 药用植物次生代谢产物生产途径的研究概述[J]. 中医药信息, 2008(1): 29—32.
[3] 罗都强,张兴,冯俊涛. 杀虫植物雷公藤研究进展[J]. 西北农业大学学报, 2000,28(3): 84—89.
[4] Dujack L W, Chen P K. The effect of precursors on Tripterygium wilfordii tissue culture [J]. Planta Medica, 1980,39(3): 281.
[5] 元英进,许晖. 前体饲喂东北红豆杉细胞强化紫杉醇生产研究[J]. 中草药, 1997,28(10): 9—12.
[6] 黄淑燕,郑郁善. 蔗糖、碳粉对雷公藤不定根生长和次生代谢产物含量的影响[J]. 武夷学院学报, 2016,35(9): 25—28.
[7] 张康健. 药用植物次生代谢[M]. 西安: 西北大学出版社, 2001: 44—49.
[8] 孙立影,于志晶,李海云,李俊波,刘洪章,林秀峰,马瑞. 植物次生代谢物研究进展[J]. 吉林农业科学, 2009,34(4): 4—10.
[9] 王莉,史玲玲,张艳霞,刘玉军. 植物次生代谢物途径及其研究进展[J]. 武汉植物学研究, 2007,25(5): 500—508.
[10] 王梦亮,任振兴,刘滇生. 前体和诱导子饲喂黄芩愈伤组织强化黄芩苷生产研究[J]. 中草药, 2007,38(1): 128—130.
[11] 杨世海,刘晓峰,果德安,郑俊华. 不同附加物对甘草愈伤组织培养中黄酮类化合物形成的影响[J]. 中国药学杂志, 2006,41(2): 96—99.
[12] Dai W H, Su Y J, Castillo C, Beslot O. Plant regeneration from in vitro leaf tissues of Viburnum dentatum L.[J]. Tissue and Organ Culture, 2011,104(2): 257—262.
[13] Onay A, Yildirim H, Tokatli Y O, Akdemir H, Suzerer V. Plant tissue culture techniques-Tools in plant micropropagation[J]. Current Opinion in Biotechnology, 2011,22(5): S130.
[14] Wackers F L, Wunderlin R. Induction of cotton extrafloral nectar production in response to herbivory does not require a herbivore-specific elicitor[J]. Entomologia Experimentalis Et Applicata, 1999,91(1): 149—154.
[15] 陶金华,濮雪莲,江曙. 内生真菌诱导子对茅苍术细胞生长及苍术素积累的影响[J]. 中国中药杂志, 2011,36(1): 27—31.
[16] Pépin M F, Chavarie C, Archambault J. Growth and immobilization of Tripterygium wilfordii cultured cells[J]. Biotechnology and Bioengineering, 1991,38(11): 1285—1291.
[17] Milling A, Babujee L, Allen C. Ralstonia solanacearum extracellular polysaccharide is a specific elicitor of defense responses in wilt-resistant tomato plants[J]. PLoS ONE, 2011,6(1): e15853.
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