基于COI基因的华南地区柑橘木虱系统发育研究

宋章蓉; 李翌菡; 欧 达; 邱宝利; 桑 文

doi:10.3969/j.issn.1009-7791.2018.01.004

亚热带植物科学 >

2018 , Vol. 47 >Issue 1: 18 - 22

DOI: https://doi.org/10.3969/j.issn.1009-7791.2018.01.004

植物生理生化与分子生物学

基于COI基因的华南地区柑橘木虱系统发育研究

宋章蓉 ,
李翌菡 ,
欧达 ,
邱宝利 ,
桑文

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(1.广东省生物农药创制与应用重点实验室/广东省害虫生物防治工程技术研究中心，广东广州 510640；2.生物防治教育部工程研究中心/华南农业大学昆虫学系，广东广州 510640)

宋章蓉，本科生，从事植物保护研究。

收稿日期: 2017-09-12

修回日期: 2017-09-29

网络出版日期: 2018-03-30

基金资助

广东省现代农业科技创新联盟建设项目(2016LM3175)；广东省现代农业产业技术体系建设推广项目(2016LM1106、2017LM1106)；广州市科技计划项目(201509010023)；广东省科技创新领军人才专项基金(2016TX03N273)

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Phylogenetic Analysis of Diaphorina citri from Southern China Based on COI Gene

SONG Zhang-rong ,
LI Yi-han ,
OU Da ,
QIU Bao-li ,
SANG Wen

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(1.Key Laboratory of Bio-Pesticide Innovation and Application/Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangzhou 510640, Guangdong China; 2.Engineering Research Center of Biological Control, Ministry of Education/Department of Entomology, South China Agricultural University, Guangzhou 510640, Guangdong China)

Received date: 2017-09-12

Revised date: 2017-09-29

Online published: 2018-03-30

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摘要

柑橘木虱Diaphorina citri是重要的柑橘害虫，其传播的黄龙病对华南地区柑橘产业造成严重危害。为了解华南地区柑橘木虱不同地理种群间的遗传多样性和进化关系，测定该地区5个地理种群线粒体COI基因900 bp序列，对不同地理种群柑橘木虱遗传距离和系统发育进行分析。结果表明，5个地理种群柑橘木虱间的遗传距离小(0.0%～0.2%)。其中，广州种群和湛江种群的柑橘木虱之间的遗传距离，以及南宁种群、桂林种群和厦门种群的柑橘木虱之间的遗传距离均为0，序列间的相似度极高。基于COI基因系统发育分析显示，位于中国的5个柑橘木虱地理种群之间分化程度较低，与国外其他地区21个种群相比，各种群间的同源性与地理距离无显著相关性。

关键词： 柑橘木虱; 线粒体COI基因; 系统发育; 遗传进化

本文引用格式

宋章蓉 , 李翌菡 , 欧达 , 邱宝利 , 桑文 . 基于COI基因的华南地区柑橘木虱系统发育研究[J]. 亚热带植物科学, 2018 , 47(1) : 18 -22 . DOI: 10.3969/j.issn.1009-7791.2018.01.004

Abstract

In order to provide references for an in-depth understanding of genetics and evolution on the citrus psyllid in southern China, mitochondrial cytochrome coxidase I (COI) genes of 5 geographic populations were cloned, and genetic distance and phylogeny were analyzed. The results showed that 5 geographic populations had a very closely genetic relationship (0.0%—0.2%). The genetic distance of Guangzhou, Zhanjiang populations was 0. The genetic distance among Xiamen, Guilin and Nanning populations was also 0. The phylogenetic analysis based on COI showed that genetic differentiation between the five populations in southern China was low. In addition, there was no obvious correlativity between the geographic distance of populations and the level of homology distance comparing with other 21 populations in other districts.

Key words： citrus psyllid; mitochondrial COI gene; phylogeny; genetic differentiation

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