Taking 174 individuals of Tetracentron sinense 26 populations as materials, 3 sampling strategies (random sampling strategy, allele preferred sampling strategy and deviation sampling strategy) were used to construct its core collection based on ISSR markers. Genetic diversity indexes were used to determine the most suiconstruction method. 180 bands were amplified14 ISSR primers, and the observed number of alleles(Na), effective number of alleles(Ne), Nei’s genetic diversity(H) and Shannon information index(I) of original germplasm were 1.6944, 1.3912, 0.2321, 0.3509 respectively, indicating the abundant genetic diversity of T. sinense was low. Genetic diversity indexes of the core collection constructedallele preferred sampling strategy were higher than those of other two strategies. 78 core collection resources of T. sinenseallele preferred sampling strategy accounted for 44.83% initial collection, and the retention rate of genetic diversity indexes(Na, Ne, H and I) were respectively 98.69%, 100.01%, 99.27%, 98.67%, which indicated that the genetic diversity and variation were well preserved. The result demonstrated that the allele preferred sampling strategy was the most appropriate method for core collection construction of T. sinense.
[1] Frankel O H. Genetic perspectives of germplasm conservation[M]// Arber W, Illmensee K, Peacock W J, Starlinger P. Genetic Manipulation: Impact on Man and Society. Cambridge: Cambridge University Press, 1984: 161—170.<br>
[2] Brown A Y H, Frankel O H, Marshall R D. The of Plant Genetic Resources[M]. Cambridge: Cambridge University Press, 1989: 136— 156.<br>
[3] 董玉琛,曹永生,张学勇,刘三才,王兰芬,游光霞,庞斌双,李立会,贾继增. 中国普通小麦初选核心种质的产生[J]. 植物遗传资源学报, 2003,4(1): 1—8.<br>
[4] 邓学斌,刘磊,闫喆,李涛,刘希艳,冯晶晶,池海娟,郑峥,李君明. 加工番茄核心种质构建及其遗传背景分析[J]. 园艺学报, 2015,42(7): 1299—1312.<br>
[5] 永文,樊丽娜,罗青文,王勤南,陈勇生,黄忠兴,刘睿,刘少谋,邓海华,李奇伟. 甘蔗细茎野生种核心种质构建[J]. 作物学报, 2013,39(4): 649—656.<br>
[6] 刘子记,曹振木,朱婕,申龙斌. 甜椒核心种质资源比较构建研究[J]. 东北农业大学学报, 2016,47(1): 21—29.<br>
[7] 崔艳华,邱丽娟,常汝镇,吕文河. 植物核心种质研究进展[J]. 植物遗传资源学报, 2003,4(3): 279—284.<br>
[8] 张春雨,陈学森,张艳敏,苑兆和,刘遵春,王延龄,林群. 采用分子标记构建新疆野苹果核心种质的方法[J]. 中国农业科学, 2009,42(2): 597—604.<br>
[9] 沈志军,马瑞娟,俞明亮,蔡志翔,许建兰. 国家果树种质南京桃资源圃初级核心种质构建[J]. 园艺学报, 2013,40(1): 125—134.<br>
[10] 李怀春. 濒危植物水青树种群生态学研究[D]. 南充: 西华师范大学硕士学位论文, 2015.<br>
[11] Wang Y F, Lai G F, Efferth T, Cao J X, Luo S D. New glycosides Tetracentron sinense and their cytotoxic activity[J]. Chemistry & Biodiversity, 2006,3(9): 1023—1030.<br>
[12] 中国科学院中国植物志编辑委员会. 中国植物志(第一卷)[M]. 北京: 科学出版社, 2004. <br>
[13] 甘小洪,田茂洁,罗雅杰. 濒危植物水青树种子的萌发特性研究[J]. 西华师范大学学报(自然科学版), 2008,29(2): 132—135. <br>
[14] 罗靖德,甘小洪,贾晓娟,谢丹,张烈. 濒危植物水青树种子的生物学特性[J]. 云南植物研究, 2010,32(3): 204—210.<br>
[15] 陈娟娟,杜凡,杨宇明,王娟. 珍稀树种水青树群落学特征及其保护研究[J]. 西南林学院学报, 2008,28(1): 12—16.<br>
[16] Hu J, Zhu J, Xu H M. Methods of constructing core collectionsstepwise clustering with three sampling strategies based on the genotypic values of crops[J]. Theoretical and Applied Genetics, 2000,101(1-2): 264— 268.<br>
[17] 刘子记,牛玉,朱婕,刘昭华,杨衍. 苦瓜核心种质资源构建方法的比较[J]. 华南农业大学学报, 2017,38(1): 31—37. <br>
[18] Yeh F C,Yang R C, Boyle T B, Ye Z H, Mao J X. POPGENE, the user-friendly shareware for population genetic analysis[A]. Molecular Biology and Biotechnology Centre, University of Alberta, Canada,1997: 10. <br>
[19] 文苗苗. 黄芩ISSR遗传多样性分析及核心种质构建方法研究[D]. 西安: 陕西师范大学硕士学位论文, 2012.<br>
[20] 张维瑞,袁王俊,尚富德. 基于AFLP分子标记的桂花品种核心种质的构建[J]. 西北植物学报,2012,32(7): 1349—1354.<br>
[21] 李自超,张洪亮,曹永生,裘宗恩,魏兴华,汤圣祥,余萍,王象坤. 中国地方稻种资源初级核心种质取样策略研究[J]. 作物学报, 2003(1): 20—24.<br>
[22] 杨汉波,张蕊,王帮顺,徐肇友,周志春. 基于SSR标记的木荷核心种质构建[J]. 林业科学, 2017,53(6): 37—46.<br>
[23] 王萱,刘晓静,邢世岩,孔倩倩,张艳辉,孙立民,高燕. 中国部分古银杏资源遗传多样性的AFLP分析及核心种质的构建[J]. 园艺学报, 2016,43(2): 249—260.<br>
[24] 马玉敏. 中国野生板栗(Castanea mollissim Blume)群体遗传结构和核心种质构建方法[D]. 泰安: 山东农业大学博士学位论文, 2009.<br>
[25] 宁宁. 我国杏核心种质的构建[D]. 南京: 南京农业大学硕士学位论文, 2009.<br>
[26] 章秋平,刘威生,刘宁,张玉萍,郁香荷,孙猛,徐铭. 普通杏(Prunus armeniaca)初级核心种质资源的构建及评价[J]. 果树学报, 2009,26(6): 819—825.<br>
[27] 郭大龙,刘崇怀,张君玉,张国海. 葡萄核心种质的构建[J]. 中国农业科学, 2012,45(6): 1135—1143.<br>
[28] 刘娟,廖康,曼苏尔?那斯尔,曹倩,江振斌,贾杨. 利用ISSR分子标记构建南疆杏种质资源核心种质[J]. 果树学报, 2015,32(3): 374—384.<br>
闽公网安备 35020602000789号