为测定阳荷Zingiber striolatum根75%乙醇粗提物及其不同浓度乙醇梯度洗脱所得样品的体外抗氧化、抑硝化、抗肝癌等生物活性，采用清除体外DPPH、ABTS自由基两种方法考察抗氧化活性；用体外清除亚硝酸盐和阻断亚硝胺合成两种方法比较抑硝化能力；用MTT方法比较其对人肝癌细胞株HepG2和SMMC7721肝癌细胞的相对存活率。结果显示，不同乙醇浓度梯度洗脱所得样品的抗氧化活性整体上比总的75%乙醇粗提物活性好。ZC-Ⅱ(75%乙醇粗提物用25%乙醇洗脱的样品)抑硝化效果最好，清除亚硝酸盐和阻断亚硝胺合成的IC50值分别为8.198 mg·mL-1、1.270 mg·mL-1。ZC-Ⅴ(75%乙醇粗提物用95%乙醇洗脱的样品)的抗肝癌效果达到95.6%，并存在剂量依赖效果。证实不同乙醇浓度洗脱所得样品的生物活性高于总粗提物活性，且生物活性部位主要集中在小极性部位。
The biological activity of anti-oxidation, anti-nitrification and anti-liver cancer in vitro was determined by measuring the extracts of Zingiber striolatum root with 75% ethanol and samples eluted by five different concentrations of ethanol. The anti-oxidant activities of six test samples were compared by scavenging DPPH and ABTS free radicals in vitro. The inhibiting nitrosation ability of the six test samples was compared by in vitro nitrite removal and blocking nitrosamine synthesis. The relative survival rates of the six samples against human hepatoma cell lines HepG2 and SMMC7721 were compared by MTT assay. The results showed that the antioxidant activity of samples eluted with different ethanol concentration gradients was better than that of the total crude extract of 75% ethanol; the inhibition of nitrification of ZC-Ⅱ(75% ethanol crude extract washed with 25% ethanol) was the best, and of which the IC50 values ??for removing nitrite and blocking nitrosamine synthesis were 8.198 mg·mL-1 and 1.270 mg·mL-1, respectively. The MTT results showed that the anti-livers effect of ZC-Ⅴ, 75% ethanol crude extract washed with 95% ethanol, could reach upto 95.6%. There was a dose-dependent effect. It was concluded that the biological activity of the samples eluted with different ethanol concentrations was higher than that of the total crude extracts, and the biological active sites were mainly concentrated in the small polar sites.
[1] 中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社, 1981: 145—146.
[2] 曹子余. 中国植物志资料(桑科)[J]. 云南植物研究, 1995,29(2): 153—154, 158.
[3] Liu B, Yang M, Yang D G, Chen H B, Li H M. Zingiber striolatum Diels derived O/N dual-doped porous carbon for high performance oxygen reduction reaction and energy storage[J]. International Journal of Hydrogen Energy, 2018,43(39): 18270—18278.
[4] Tian M Y, Liu T T, Wu X H, Hong Y, Liu X L, Lin B, Zhou Y. Chemical composition, antioxidant, antimicrobial and anticancer activities of the essential oil from the rhizomes of Zingiber striolatum Diels[J]. Natural Product Research, Doi:10.1080/14786419.2018.1544979.
[5] 王军民,周凡蕊,陈川云,水星,华燕. GC-MS法测定阳荷花挥发油的成分[J]. 天然产物研究与开发, 2012,24(7): 916—919.
[6] Chen T, Cai J, Ni J, Yang F. An UPLC-MS/MS application to investigate chemical compositions in the ethanol extract with hypoglycemic activity from Zingiber striolatum Diels[J]. Journal of Chinese Pharmaceutical Sciences, 2016,25(2): 116—121.
[7] 陈仕学,郁建平,陈美航,沈家国. 梵净山野生阳荷红色素的提取及其抗氧化性研究[J]. 食品工业科技, 2012(9): 276—280.
[8] 蔡依,郭百臻,徐津林,李肖,段金辉,阮金兰. 阳荷不同极性部位体外抑菌活性研究[J]. 食品研究与开发, 2018,39(19): 36—39.
[9] 俞永琼,陆宇惠,郭世民,刘因华,夏亮,马佶,马克坚,曹玺. 阳荷根药材质量标准初步研究[J]. 云南中医中药杂志, 2012,33(7): 61—63.
[10] 安徽省革委会卫生局《安徽中草药》编写组. 安徽中草药[M]. 合肥: 安徽人民出版社, 1975: 401.
[11] 马佶,王彧,吴秋瑜,钮奥,郭世民,俞永琼. 阳荷药材化学成分的预试验[J]. 云南中医中药杂志, 2012,33(4): 66—67.
[12] 王丽娜,郭世民,高敏. 植物药阳荷根的急性毒性研究[J]. 云南中医中药杂志, 2010,31(12): 64.
[13] Sonklin C, Laohakunjit N, Kerdchoechuen O. Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates[J]. PeerJ, 2018,6(7): 17—36.
[14] El-kalyoubi S A. Synthesis and anticancer evaluation of some novel pyrimido[5,4-e][1,2,4] triazines and pyrazolo[3,4-d] pyrimidine using DMF-DMA as methylating and cyclizing agent[J]. Chemistry Central Journal, 2018,12(1): 64—78.
[15] Jiao Y, Kilmartin P A, Fan M T, Quek S Y. Assessment of phenolic contributors to antioxidant activity of new kiwifruit cultivars using cyclic voltammetry combined with HPLC[J]. Food Chemistry, 2018,46(6): 77—85.
[16] 赵玉静,李建宽,张鑫,高建平. 番石榴叶黄酮类化学成分及其抗氧化活性研究[J]. 中国中药杂志, 2018,43(4): 760—765.
[17] 黄晓冬,李裕红,戴聪杰,李元跃,黄晓昆. 红树植物桐花树提取物清除亚硝酸盐与阻断亚硝胺合成的体外评价[J]. 中国食品学报, 2015,15(9): 15—22.
[18] Choi S Y, Chung M J, Seo W D, Shin J H, Shon M Y, Sung N J. Inhibitory effects of Orostachys japonicus extracts on the formation of N-nitrosodimethylamine[J]. Journal of Agricultural and Food Chemistry, 2006,54(16): 6075—6078.
[19] 傅茂润,陈庆敏,茅林春. 黄花菜提取物对亚硝化反应的抑制能力研究[J]. 食品科学, 2009,30(15): 114—119.
[20] Liao E, Xu Y S, Jiang Q X, Xia W S. Effects of inoculating autochthonous starter cultures on N-nitrosodimethylamine and its precursors formation during fermentation of Chinese traditional fermented fish[J]. Food Chemistry, 2019(271): 174—181.
[21] Mansour D F, Abdallah H M, Ibrahim B M, Hegazy R R, Esmail R S, Abdelsalam L O. The carcinogenic agent diethylnitrosamine induces early oxidative stress, inflammation and proliferation in rat liver, stomach and colon: Protective effect of ginger extract[J]. Pharmacognosy Magazine, 2019,20(8): 2551—2561.
[22] 马小妮. 高良姜化学成分及其抗癌活性研究[D]. 广州: 广东药科大学硕士学位论文, 2016.
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