以‘云薯505’马铃薯(Solanum tuberosum ‘Yunshu 505’)为材料，测定马铃薯块茎发育初期四个阶段茉莉酸含量，并以叶面喷施方式，研究茉莉酸甲酯对马铃薯生长和块茎产量的影响。结果表明，马铃薯块茎膨大过程中，茉莉酸的积累水平不断升高。在微型薯生产中，使用100 μmol·L-1茉莉酸甲酯在结薯期以不同频率喷施叶面，测量并统计植株、块茎性状及产量变化。结果表明，与对照(CK)相比，1次·d-1处理茎粗增加36.1%，2次·d-1处理的叶绿素含量降低20.1%。此外，植株的叶色、茎色、花色、株型等生长性状及块茎大小整齐度、薯形、皮色、肉色、薯皮类型、芽眼深浅、裂薯率、大薯空心率等块茎性状在各组间没有显著差异。2次·d-1、1次·d-1、1次·2d-1、CK四种处理的植株存活率分别为45.57%、100.00%、100.00%、87.29%；前三种喷施频率处理折合产量较CK分别增加-15.61%、8.77%、12.11%。综合分析，马铃薯在块茎形成初期茉莉酸积累水平不断升高，以1次·2d-1频率叶面喷施100 μmol·L-1茉莉酸甲酯，马铃薯微型薯的产量增加最大且不影响生长。
In this study, using greenhouse-grown Solanum tuberosum ‘Yunshu 505’ plants as experimental material, we measured the levels of jasmonic acid in four early tuberization stages, and explored the effect of foliar sprayed methyl jasmonate on the growth and yield of minituber. The results indicated that the levels of jasmonic acid were increased as tubers enlarged. During minituber cultivation, 100 μmol·L-1 methyl jasmonate (MeJA) solution or mock solution (CK) was sprayed by different frequencies on leaf surface, and the statistical changes in agronomic trait and yield were analyzed. Our results showed that compared with CK, the average stem diameter with once per day treatment was increased by 36.1%, and the chlorophyll content in twice per day treatment was reduced by 20.1%. Moreover, no significant difference was detected after MeJA treatment between groups in either morphological traits including leaf color, stem color, flower color, plant architecture, or tuber traits including tuber uniformity, tuber shape, peel color, flesh color, peel type, eye depth, crack tuber rate, large-sized tuber hollow rate. Moreover, the plant survival rates in twice per day, once per day, once every 2 days, and CK treatment were 45.57%, 100.00%, 100.00%, and 87.29%, respectively. Compared with CK, the equivalent yields in MeJA treatment above were increased by -15.61%, 8.77%, and 12.11%, respectively. Taken together, our results showed that the levels of jasmonic acid were increased at the early stages of tuberization, and the potato plants which were foliar sprayed with 100 μmol·L-1 methyl jasmonate once every 2 days had the maximal yield without the cost of growth.
[1] 全峰,张爱霞,曹先维. 植物激素在马铃薯块茎形成发育过程中的作用[J]. 中国马铃薯, 2002,16(1): 29–32.
[2] Gibson S I. Sugar and phytohormone response pathways: navigating a signalling network[J]. Journal of Experimental Botany, 2004,55(395): 253–264.
[3] 刘丹,徐梦薇,胡月清,王若仲,童建华,肖浪涛. 马铃薯块茎发育期主要植物激素的动态变化[J]. 分子植物育种, 2019,17(6): 1998–2003.
[4] Abdala G, Castro G, Miersch O, Pearce D. Changes in jasmonate and gibberellin levels during development of potato plants (Solanum tuberosum)[J]. Plant Growth Regulation, 2002,36(2): 121–126.
[5] Aksenova N P, Konstantinova T N, Golyanovskaya S A, Sergeeva L I, Romanov G A. Hormonal regulation of tuber formation in potato plants[J]. Russian Journal of Plant Physiology, 2012,59(4): 451–466.
[6] Wasternack C, Hause B. Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany[J]. Annals of Botany, 2013,111(6): 1021–1058.
[7] Yoshihara T, Omer E A, Koshino H, Sakamura S, Kikuta Y, Koda Y. Structure of a tuber-inducing stimulus from potato leaves (Solanum tuberosum L)[J]. Agricultural and Biological Chemistry, 1989,53(10): 2835–2837.
[8] Nam K H, Kong F, Matsuura H, Takahashi K, Nabeta K, Yoshihara T. Temperature regulates tuber-inducing lipoxygenase-derived metabolites in potato (Solanum tuberosum)[J]. Journal of Plant Physiology, 2008,165(2): 233–238.
[9] Kolomiets M V, Hannapel D J, Chen H, Tymeson M, Gladon R J. Lipoxygenase is involved in the control of potato tuber development[J]. Plant Cell, 2001,13(3): 613–626.
[10] Pelacho A M, Mingo-Castel A M. Jasmonic acid induces tuberization of potato stolons cultured in vitro[J]. Plant Physiology, 1991,97(3): 1253–1255.
[11] 甘立军,曾晓春,周燮. 茉莉酸类与植物地下贮藏器官的形成[J]. 植物学通报, 2001,18(5): 546–553.
[12] 陈大清,王雪英,李亚男. 水杨酸和茉莉酸甲酯对试管马铃薯形成的影响[J]. 华中农业大学学报, 2005,24(1): 74–78.
[13] 秦忠群,王季春. 赤霉素(GA3)与茉莉酸甲酯(MeJA)对雾培马铃薯内源激素与生长发育的影响[J]. 中国马铃薯, 2006,20(1): 5–11.
[14] Fernie A R, Willmitzer L. Molecular and biochemical triggers of potato tuber development[J]. Plant Physiology, 2001,127(4): 1459–1465.
[15] Luo J, Wei K, Wang S, Zhao W, Ma C, Hettenhausen C, Wu J, Cao G, Sun G, Baldwin I T, Wu J, Wang L. COI1-regulated hydroxylation of jasmonoyl-L-isoleucine impairs nicotiana attenuata’s resistance to the generalist herbivore Spodoptera litura[J]. Journal of Agricultural and Food Chemistry, 2016,64(14): 2822–2831.
[16] 张东昱,夏叶,张文斌,成军花,李文伟. 叶面喷施磷酸二氢钾对加工型马铃薯生长的影响[J]. 中国马铃薯, 2010,24(5): 298–300.
[17] 申惠波. 马铃薯叶面喷施植物生长调节剂对植株和产量的影响[J]. 中国马铃薯, 2007(4): 209–211.
[18] 苏云松, 郭华春, 陈伊里. 马铃薯叶片SPAD值与叶绿素含量及产量的相关性研究[J]. 西南农业学报, 2007(4): 690–693.
[19] Wu J, Wang L, Baldwin I T. Methyl jasmonate-elicited herbivore resistance: does MeJA function as a signal without being hydrolyzed to JA?[J]. Planta, 2008,227(5): 1161–1168.
[20] Zhang Y, Wang Y, Wei H, Li N, Tian W, Chong K, Wang L. Circadian evening complex represses jasmonate-induced leaf senescence in Arabidopsis[J]. Molecular Plant, 2018,11(2): 326–337.
[21] Aubry S, Fankhauser N, Ovinnikov S, Pruzinska A, Stirnemann M, Zienkiewicz K, Herrfurth C, Feussner I, Hortensteiner S. Pheophorbide a may regulate jasmonate signaling during dark-induced senescence[J]. Plant Physiology, 2020,182(2): 776–791.
[22] Hu Y, Jiang Y, Han X, Wang H, Pan J, Yu D. Jasmonate regulates leaf senescence and tolerance to cold stress: crosstalk with other phytohormones[J]. Journal of Experimental Botany, 2017,68(6): 1361–1369.
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