为了研究大蓟(Cirsium japonicum)紫外光谱的显著鉴别特征,以快速、准确地鉴别正品大蓟,采用多溶剂紫外光谱法分析福建省不同地区的自采大蓟和市售18批不同厂家商品大蓟在无水乙醇、氯仿及石油醚溶剂中紫外特征差异,并采用主峰值(含肩峰值)及主谷值的均值加方差归纳比较特征。结果表明,自采大蓟在无水乙醇溶剂中表现为“谷—峰—谷—峰”,即在273±3 nm、326±2 nm处的峰值和250±2 nm、298±1 nm处的谷值具有显著鉴别意义;在氯仿溶剂中,表现为“峰—谷—峰”,即在245±3 nm、273±4 nm处的峰值和260±3 nm处的谷值具有显著鉴别意义;在石油醚溶剂中表现为“肩峰—谷—峰”,即在233±3 nm处的肩峰、265±5 nm处的峰值和257±5 nm处的谷值具有显著鉴别意义。紫外光谱的峰谷特征可用于大蓟鉴别。
The identifiable characteristics of Cirsium japonicum was studied by UV spectrum for a quick and accurate identification. Multi-solvent ultraviolet spectroscopy was used to analyze the difference of ultraviolet characteristics between self-collected C. japonicum from different areas of Fujian province and 18 batches of commercial C. japonicum from different manufacturers in anhydrous ethanol, chloroform and petroleum ether solvents, and the characteristics by mean plus variance of the main peak value (shoulder peak value) and the mean valley value were induced. The self-collected C. japonicum shows ‘valley-peak-valley-peak’ in anhydrous ethanol solvent: the peak value at 273±3 nm, 326±2 nm and the valley value at 250±2 nm, 298±1 nm, which have significant differential significance; In chloroform solvent, it shows ‘peak-valley-peak’: the peak value at 245± nm, 273±4 nm and the valley value at 260±3 nm, which have significant discrimination significance; In petroleum ether solvent, it shows ‘shoulder peak-valley-peak’: the peak value at 233±3 nm, 265±5 nm and the valley value at 257±5 nm, which have significant discrimination significance. The peak and valley characteristic induction of UV spectrum can be used to identify C. japonicum.
[1] 国家药典委员会. 中华人民共和国药典: 一部 (2020年版)[M]. 北京: 中国医药科技出版社, 2020: 26.
[2] 赵彧,邱明阳,刘玉婷,杨洋,睢博文,严铭铭,邵帅,徐东铭. 大蓟化学成分及药理活性研究进展[J]. 中草药, 2017,48(21): 4584–4590.
[3] 王卫明,刘亮,彭慧,张贵君. 大蓟、小蓟功效的考证和现代研究[J]. 中国现代应用药学, 2019,36(1): 81–84.
[4] 北京药品生物制品检定所,中国科学院植物研究所. 中药鉴别手册(第1册)[M]. 北京: 科学出版社, 1972: 26–30.
[5] 丁振飞,黄和平,杨青山,金捷凯,陈梦轩,吴德玲,章鹏飞. 中药大蓟及其2种伪品的微性状鉴别[J]. 安徽中医药大学学报, 2020,39(4): 78–82.
[6] 史春亮,邢益智,赵冰玉,冶文静,尹文萱. 紫外光谱法在复方中药研究中的应用[J]. 光谱学与光谱分析, 2018,38(S1): 317–318.
[7] 黎创幸,黄玉兴,岑志芳. 紫外谱线组扫描法鉴别大青叶药材的真伪[J]. 天津药学, 2017,29(4): 17–19,42.
[8] 刘训红,王玉玺主编. 中药材光谱鉴别[M]. 上海: 第二军医大学出版社, 2001: 32.
[9] 车苏容. 一种鉴别当归、欧当归及独活的方法[P]. CN106918568B, 2020-04-28.
[10] 周文序,田珍. 中药大、小蓟中柳穿鱼甙和芦丁的薄层扫描法定量分析[J]. 药物分析杂志, 1994,14(6): 39–40.
[11] 周文序,田珍. 中药大小蓟的黄酮类成分的分离和鉴定[J]. 北京医科大学学报, 1994,26(4): 309.
[12] 郝晶晶,张府君,甄会贤,卫甲鑫. HPLC法同时测定大蓟及其炮制品中7种成分含量[J]. 中药材, 2020(5): 1117–1120.