针对南方低山丘陵区种植重楼存在资源混杂及可借鉴文献少等的问题,本文对搜集的120份重楼种质资源在福建省的物候期、病害发生和农艺性状进行观测,并对生长较好的资源采用HPLC法对重楼皂苷进行测定,利用主成分分析和权重评分法开展七叶一枝花种质资源适应性评价。结果表明搜集到的西南地区包括云贵川重楼资源在福建省的生育期不完整,物候期及农艺性状等指标较差,而另外有49份表现良好的为七叶一枝花资源,其中符合《中国药典》重楼皂苷含量的占55%。七叶一枝花资源的病害、农艺性状和重楼皂苷含量存在着广泛的变异,南方低山丘陵区种植重楼应选择当地或相似经纬度及地形地貌地区的七叶一枝花优异资源。七叶一枝花种质资源的评价,是以适应性、有效成分及农艺性状为主成分来进行权重评分,总得分越高则适应性越强。
In order to solve the problems of mixed resources and few references in the process of planting Paridis Rhizoma in the low mountain and hilly areas of the south, this study observed the phenology, disease occurrence and agronomic characteristics of 120 Paridis Rhizoma resources collected in Fujian Province. And for the better-growing resources, the Paridis Rhizoma saponin was determined by HPLC method, and the correlation analysis and cluster analysis were performed by DPS software. The results showed that the collected resources of Paridis Rhizoma in the southwest region, including Yunnan, Guizhou and Sichuan, had incomplete growth periods in Fujian Province, and poor phenological periods and agronomic traits. Among 49 resources of Paridis Rhizoma, the content of saponins of Paridis Rhizoma in accordance with the Chinese Pharmacopoeia accounted for 55%. Paridis Rhizoma has wide variations in disease, agronomic traits and saponins content of Paridis Rhizoma. For planting heavy buildings in low mountain and hilly areas, the excellent resources of Paris polyphylla var. chinensis in the local or similar latitude, longitude and topography should be selected. The germplasm resources of P. polyphylla var. chinensis are scored based on the weight of adaptability, active ingredients and agronomic traits as the main components. The higher the total score, the more adaptable. This study provides reference for the screening and evaluation of P. polyphylla var. chinensis germplasm resources.
[1] 蒋露,康利平,刘大会,彭华胜,谢晋陈敏. 历代本草重楼基原考[J]. 中国中药杂志, 2017,42(18): 3469–3473.
[2] 陶爱恩,赵飞亚,李若师,钱金栿,夏从龙. 重楼产业现状及发展对策[J]. 中草药, 2020, 51(18): 4809–4815.
[3] 李恒,苏豹,张兆云,杨宇明. 中国重楼资源现状评价及其种植业的发展对策[J]. 西部林业科学, 2015, 44(03): 1–7.
[4] 苏海兰,周先治,李希,陈敏健,卓景泰,唐建阳,单寄坪,林映从. 七叶一枝花种子萌发不同阶段生理生化变化研究[J].中草药,2017,48(22):4755–4763.
[5] 黎华君,曾令祥,彭慧元. 贵阳市百宜野生重楼种质资源的调查[J]. 贵州农业科学, 2011, 39(1): 28–30.
[6] 尹鸿翔,张浩. 濒危民族药重楼种质资源调查及质量评价研究[J]. 时珍国医国药, 2009, 20(11): 2863–2865.
[7] 苏泽春,王泽清,陈翠. 运用聚类分析与TOPSIS法综合评价云南重楼种质资源[J]. 贵州农业科学, 2015, 43(4): 16–19+24.
[8] 郑梅霞,陈宏,苏海兰,朱育菁. 不同重楼资源的重楼皂苷含量比较及土壤养分的相关性分析[J]. 福建农业科技, 2020,(8):58–66.
[9] 陈士林. 中国药材产地生态适宜性区划[M]. 2011.
[10] 陈铁柱,文飞燕,张涛. 21个产地七叶一枝花中皂苷类成分的评价[J]. 中成药, 2017, 39(011): 2345–2350.
[11] 李海涛,罗先文,管燕红. 云南省不同地区滇重楼皂苷含量的对比及影响因子分析[J]. 中国中药杂志, 2014(05): 803–806.
[12] 周爱存,闫道良,黄妍. 浙江七叶一枝花种质资源的化学评价[J]. 中国现代应用药学, 2015, (09): 1065–1069.
[13] 梁社往,张世华,何忠俊. 不同地理种源滇重楼叶片碳氮代谢关键生理生化指标研究[J]. 云南农业大学学报, 2015, 30(5): 771–776.
[14] 邹亮,周浓,张海珠. HPLC测定不同产地滇重楼中的4种重楼皂苷[J]. 华西药学杂志, 2009, 24(5): 521–523.
[15] 叶方,黄良永,陈黎,胡培,朱敏,孙良学,雷攀. 武当山区人工种植重楼中9种重楼皂苷含量分析[J]. 中南药学, 2016,14(11): 1244–1248.
[16] 李晨,刘立敏,杨敏,李海峰. 多叶重楼与云南重楼中4种重楼皂苷的积累差异[J]. 中成药, 2017, 39(7): 1454–1459.