采用水蒸气蒸馏法和气相色谱-质谱联用技术(GC-MS)分析阔叶百里香(Thymus pulegioides)鲜、干茎叶精油含油率、化学组成及其相对含量的差异，并比较精油对链格孢(Alternaria alternata)、粉红单端孢(Trichothecium roseum)和意大利青霉(Penicillium italicum)的抑制效果。鲜、干茎叶含精油率分别为0.53%、0.37%，分别鉴定出35、40种化合物，其主要化学成分相同，均含有百里香酚(鲜样31.30%，干样26.82%)、麝香草酚甲醚、右旋龙脑和邻伞花烃等，只是相对含量略有差异；在抑菌实验中，百里香精油对链格孢菌的抑制作用最为明显，其次是意大利青霉和粉红单端孢。鲜、干阔叶百里香精油的组分较为接近，相对含量略有差异；阔叶百里香精油对3种真菌具有较好的抑制效果，且鲜样精油的抑菌效果好于干样精油。
The essential oil from fresh and dry leaves and stems of Thymus pulegioides was extracted, and their components were detected and identified, and their antibacterial effects were analyzed, so as to provide the basis for the development and application of thyme in this region. Contents of essential oil from fresh and dry thyme were analyzed and compared, by steam distillation extraction and GC-MS. And antibacterial effects of the two essential oils from fresh and dry samples on Alternaria alternate, Trichothecium roseum and Penicillium italicum were compared. The essential oil contents in fresh and dry samples were 0.53% and 0.37%, and total of 35 and 40 compositions were identified, respectively. They had similar main compositions: Thymol(fresh 31.30%, dry 26.82%), 3-methoxy-p-cymene, Borneol and o-Cymene and so on, and these compositions were slightly different in contents. In addition，the inhibitory effect of thyme essential oil on Alternaria alternate was the most significant, the second was Penicillium italicum and Trichothecium roseum. The compositions of essential oils from fresh and dry samples were similar, and the relative contents were slightly different. The essential oil of thyme had a good inhibitory effect on 3 species of fungi, and the antibacterial effect of essential oil of fresh samples was better than that of dry ones.
[1] 王娣,程柏,丁莉,任茂生. 百里香精油的提取工艺及化学成分分析[J]. 中国调味品, 2019,44(7): 76–80.
[2] 中国科学院中国植物志编辑委员会. 中国植物志(第66卷)[M]. 北京: 科学出版社, 1977: 250–259.
[3] Victor R, David J M, Maria G C, Lynne M. Effect of ripening inhibitor type on formation, stability, and antimicrobial activity of thyme oil nanoemulsion[J]. Food Chemistry, 2018,45: 104–111.
[4] Pasquale M F, Mariana M, Matteo A D N, Maria R C, Milena S．Evaluating in vitro antimicrobial activity of thymol toward hygiene-indicating and pathogenic bacteria[J]. Journal of Food Protection, 2007,70(2): 425–431.
[5] 李佳,张富新,张拥军. 百里香提取物在中式香肠中的抗菌及抗氧化性能的研究[J]. 中国食品学报, 2007(3): 107–111.
[6] 孙震晓,马清温. 百里香活性成分及药理作用研究[J]. 中西医结合学报, 2009,7(2): 175–178.
[7] Gruenwald J, Graubaum H J, Busch R. Efficacy and tolerability of a fixed combination of thyme and primrose root in patients with acute bronchitis. A double-blind, randomized, placebo-controlled clinical trial[J]. Arzneimittel-Forschung, 2005,55(11): 669–676.
[8] 孙震晓,张英慧,程霜,马清温,郭善利,张金保. 中药地椒乙醇提取物对人白血病细胞增殖的抑制作用[J]. 中西医结合学报, 2005,3(5): 382–385.
[9] Meriem B, Bo J, Consolate N, Tang J. Effect of lavender and thyme incorporation in sunflower seed oil on its resistance to frying temperatures[J]. Food Research International, 2007,40: 341–346.
[10] Mohammad H, Ester S, Anna M D P, Vanni C. GC/MS evaluation of thyme (Thymus vulgaris L.) oil composition and variations during the vegetative cycle[J]. Journal of Pharmaceutical & Biomedical Analysis, 2002,29(4): 691–700.
[11] 赵亚珠,郝晓秀,孟婕,张然,刘光发. 百里香精油抗菌包装纸箱对草莓保鲜效果的影响[J]. 食品与发酵工业, 2020,46(11): 258–263.
[12] Natalia M, Lillian B, Celestino S, Sonia S, Mariana H, Isabel F. Decoction, infusion and hydroalcoholic extract of cultivated thyme: Antioxidant and antibacterial activities, and phenolic characterisation[J]. Food Chemistry, 2015,167: 131–137.
[13] 梁锐,赵雅清,成钟,魏硕明,陈秀红,岳晓华,杜俊明,薛慧清. 响应面法优化五脉百里香挥发油超临界CO2萃取条件及抑菌活性分析[J].辽宁中医药大学学报, 2017,19(4): 46–49.
[14] 裴海闰,韩笑,曹学丽. 百里香精油的成分分析及其抗氧化和抑菌活性评价[J]. 中国食品学报, 2011,5: 182–188.
[15] 陈品品,蔡锦玲,马晓娟,蓝波妙,吴辉煌,庄卫东. 不同百里香精油成分的GC–MS分析[J]. 热带生物学报, 2020,11(4): 1–9.
[16] 黄健,马莉,姚雷,吴亚妮. 七种百里香精油的主成分分析及其化学型[J]. 上海交通大学学报(农业科学版), 2009,27(3): 206–209.
[17] 李建国,杨莉,徐世千,李晓东. NaCl和壳聚糖对离体培养百里香精油的影响[J]. 热带作物学报, 2011,32(10): 1910–1914.
[18] 徐世千,李晓东,张建国. 不同方法提取组培百里香精油质量及成分的比较分析[J]. 植物科学学报, 2013,31(6): 609–615.
[19] 冯辽辽,宗荣荣,刘婉如. 地椒研究进展[J]. 黑龙江农业科学, 2019(4): 162–164.
[20] 许彩虹,韦杰. 不同产地仙草中挥发性风味成分比较分析[J]. 现代食品, 2016(11): 1–5.
[21] 刘树文. 合成香料技术手册[M]. 北京: 中国轻工业出版社, 2009: 2–9.
[22] 张萍,王平,石超峰,魏琴,杜永华,周黎军,殷中琼,贾仁勇. 油樟油主成分对几种常见病原菌的抑菌活性研究[J]. 四川农业大学学报, 2013,31(4): 393–397.
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