以无菌萌发的天门冬(Asparagus cochinchinensis)种子胚轴为外植体，研究植物生长调节物质种类及浓度对愈伤组织诱导、丛生芽分化和植株再生的影响，建立其离体快速繁殖技术。结果表明，愈伤组织诱导的适宜培养基为MS + 6-BA 1.0 mg·L-1 + NAA 0.5 mg·L-1，诱导率95.6；愈伤组织增殖培养基为MS + 6-BA 0.5 mg·L-1 + NAA 2.0 mg·L-1，增殖倍数为9.7；丛生芽诱导培养基为MS + 6-BA 0.5 mg·L-1 + NAA 0.1 mg·L-1 + KT 0.1 mg·L-1，诱导率为91.1；适宜的壮苗培养基为MS + 6-BA 0.2 mg·L-1 + IAA 1.0 mg·L-1；适宜的生根培养基为1/2MS + NAA 2.0 mg·L-1，生根率达84.4。本研究为构建天门冬药材产业化所需种苗生产技术体系奠定了基础。
李 林
,
李 扬
,
张明生
,
彭思静
,
吴尚浇
,
贾 娜
,
刘 智
,
于晓松
,
杨朝雄
. 天门冬离体快繁技术[J]. 亚热带植物科学, 2021
, 50(01)
: 46
-50
.
DOI: 10.3969/j.issn.1009-7791.2021.01.008
Into establish the rapid propagation technique in vitro of Asparagus cochinchinensis, the hypocotyls in seedsaseptic sprouting were used as explants, the effects of different plant growth regulators on callus induction, shoots differentiation and plants regeneration were studied. The results showed that the suimedium for callus induction was MS + 6-BA 1.0 mg·L-1 + NAA 0.5 mg·L-1, and the induction rate was 95.6; The proliferation medium of callus was MS + 6-BA 0.5 mg·L-1 + NAA 2.0 mg·L-1, and the proliferation multiple was 9.7; The induction medium of clustered buds was MS + 6-BA 0.5 mg·L-1 + NAA 0.1 mg·L-1 + KT 0.1 mg·L-1, the induction rate was 91.1; The suimedium for strong seedlings was MS + 6-BA 0.2 mg·L-1 + IAA 1.0 mg·L-1; The suirooting medium was 1/2 MS + NAA 2.0 mg·L-1, the rooting rate reached 84.4. This study laid a foundation for the technical system construction of seedlings production in the industrialization of A. cochinchinensis.
[1] 中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社, 2004. [2] Kim J E, Park J W, Kang M J, Choi H J, Bae S J, Choi Y S, Lee Y J, Lee H S, Hong J T, Hwang D Y. Anti-inflammatory response and muscarinic cholinergic regulation during the laxative effect of Asparagus cochinchinensis in loperamide-induced constipation of SD rats[J]. International Journal of Molecular Sciences, 2019,20(4): 946. [3] Rajasekhar A, Peddanna K, Vedasree N, Munirajeswari P, Nagaraju N, Badri K R, Chippada A R. Antidiabetic activity of root tubers of Asparagus gonoclados Baker in streptozotocin induced diabetic rats[J]. Journal of Ethnopharmacology, 2019,242: 1–9. [4] Wang G H, Lin Y M, Kuo J T, Lin C P, Chang C F, Hsieh M C, Cheng C Y, Chung Y C. Comparison of biofunctional activity of Asparagus cochinchinensis (Lour.) Merr. extract before and after fermentation with Aspergillus oryzae[J]. Journal of Bioscience and Bioengineering, 2019,127(1): 59–65. [5] 国家药典委员会. 中华人民共和国药典(第一部)[M]. 北京: 中国医药科技出版社, 2020. [6] 中华本草编委会. 中华本草[M]. 上海: 上海科技出版社, 2004. [7] 赵明. 我国天门冬研究的概况及展望[J]. 内江师范学院学报, 2005,20(6): 52–55. [8] 姚元枝,欧立军. 不同土壤对天门冬生长的影响[J]. 中药材, 2015,38(2): 234–236. [9] 张明生. 贵州主要中药材规范化种植技术[M]. 北京: 科学出版社, 2013. [10] 葛锦华,曾桂萍. 天冬愈伤诱导培养基的筛选[J]. 贵州农业科学, 2010,38(2): 16–18. [11] 刘玉艳,于凤鸣,靳晓翠. 天门冬种子萌发特性研究[J]. 种子, 2007,26(9): 35–38. [12] 全妙华,欧立军,贺安娜,孙榕, 于广游. 天门冬组培快繁体系研究[J]. 中草药, 2012,43(8): 1599–1603. [13] 龚振辉,申书兴. 植物组织培养[M]. 北京: 化学工业出版社, 2013. [14] 潘丽梅,马小军,韦树根.王晓峰,柯芳. 天门冬组织培养条件的优化[J]. 湖北农业科学, 2012,51(19): 4396–4398. [15] 谢璇,许轲,谢闽新,朱元娣. 苹果茎尖培养快繁体系的优化[J]. 植物生理学报, 2015,51(12): 2152–2156. [16] 王一诺,韦莹,秦双双,李翠. 植物生长调节素对黑果菝葜组织培养的影响[J]. 中药材, 2017,40(8): 1773–1776.
闽公网安备 35020602000789号