植物生理生化与分子生物学

烟草Trihelix转录因子家族鉴定及表达分析

  • LI Dong-cheng ,
  • LI Kui-yin ,
  • WEI Xing-qi ,
  • DUAN Li-li ,
  • MO Ze-jun ,
  • LIU Ren-xiang
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  • (1. 贵州大学烟草学院/贵州省烟草品质研究重点实验室,贵州 贵阳 550025;2. 安顺学院农学院,贵州 安顺 561000;3. 黔南州烟草公司长顺县分公司,贵州 长顺 550700)

收稿日期: 2022-01-02

  录用日期: 2022-01-25

  网络出版日期: 2022-05-17

Identification and Expression Analysis of Trihelix Transcription Factor Family in Tobacco

  • 李栋成,李魁印,韦兴启,段丽丽,莫泽君,刘仁祥
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  • (1. Tobacco College of Guizhou University / Key Laboratory of Tobacco Quality Research of Guizhou Province, Guiyang 550025, Guizhou China; 2. Agricultural College of Anshun College, Anshun 561000, Guizhou China; 3. Changshun County Branch of Qiannan Tobacco Company, Changshun 550700, Guizhou China)

Received date: 2022-01-02

  Accepted date: 2022-01-25

  Online published: 2022-05-17

摘要

运用生物信息学分析方法,通过对烟草(Nicotiana tabacum)全基因组数据中的Trihelix家族基因的理化性质、染色体分布、染色体共线性、基序组成、基因结构、顺式作用元件及其低温处理下的表达进行完整的鉴定分析,初步解析烟草Trihelix家族成员的结构与功能。从普通烟草中鉴定出32个Trihelix基因,分为GT-1、GT-2、GTγ、SH4、SIP1等5个亚家族;32个NtTH基因有着10种分布不均匀的基序,且有不同的基因结构,但在同一个亚家族内有相似性;烟草与番茄(Lycopersicon esculentum)、拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)存在同源的Trihelix基因,普通烟草Trihelix家族自身也存在共线性基因;鉴定出13种不同类型的顺式元件,主要与非生物胁迫、激素和生长发育有关,其中ABRE和MeJA是最多的两个顺式元件,表明NtTH基因的功能主要与非生物胁迫和生长发育响应调控有关;烟草NtTH3和NtTH16等11个基因在对低温胁迫的响应方面有着重要作用。本研究挖掘到与烟草抗冷有关的基因,为烟草抗冷性遗传改良提供了理论依据。

本文引用格式

LI Dong-cheng , LI Kui-yin , WEI Xing-qi , DUAN Li-li , MO Ze-jun , LIU Ren-xiang . 烟草Trihelix转录因子家族鉴定及表达分析[J]. 亚热带植物科学, 2022 , 51(1) : 1 -12 . DOI: 10.3969/j.issn.1009-7791.2022.01.001

Abstract

Tobacco is a kind of temperature-loving economic crop. Screening and utilization of cold-resistant genes plays an important role in the growth and development, yield and quality of tobacco. In this study, bioinformatics analysis was used to identify and analyze the physical and chemical properties, chromosome distribution, chromosome collinearity, motif composition, gene structure, cis-acting elements and expression of Trihelix family genes in tobacco genome data, and the structure and function of tobacco Trihelix family members were preliminarily analyzed. Thirty-two Trihelix genes were identified from common tobacco, which were divided into 5 subfamilies: GT-1, GT-2, GTγ, SH4 and SIP1. Thirty-two NtTH genes had 10 different motifs and different gene structures, but were similar in the same subfamily. There were homologous Trihelix genes in tobacco, tomato, Arabidopsis and rice, and there were collinear genes in the Trihelix family of common tobacco. Thirteen different types of cis elements were identified, which were mainly related to abiotic stress, hormone and growth and development. ABRE and MeJA were the two most cis elements, indicating that the function of NtTH gene was mainly related to abiotic stress and the regulation of growth and development response. Among them, 11 genes, such as tobacco NtTH3 and NtTH16, played an important role in response to low temperature stress. In this study, the genes related to cold resistance of tobacco were found, which provided a theoretical basis for the genetic improvement of cold resistance and stress resistance of tobacco.

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