植物生理生化与分子生物学

外施GA3对茉莉花主要萜类物质及其相关基因表达的影响

  • ZHAO Wei-shu ,
  • WAN Chao ,
  • HU Li ,
  • ZHANG Qu ,
  • YUAN Yuan
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  • (福建农林大学园艺学院,福建 福州 350002)

收稿日期: 2022-06-25

  录用日期: 2022-08-04

  网络出版日期: 2023-01-17

基金资助

福建农林大学大学生创新创业训练计划项目(202110389187);福建农林大学科技创新专项基金项目(CXZX2020135C)

Changes of Major Terpenoids Contents and Related Genes’ Expression Level in Jasmine after GA3 Treatment

  • 赵炜淑,万 超,胡 莉,张 蕖,袁 媛
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  • (College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China)

Received date: 2022-06-25

  Accepted date: 2022-08-04

  Online published: 2023-01-17

摘要

对茉莉花喷施100 μmol·L–1赤霉素(GA3),分析赤霉素对茉莉花主要香气组分及其合成相关结构基因和转录因子表达水平的影响。结果显示,外施GA3可提高花朵开放过程中总香气成分以及芳樟醇、法呢烯含量,与此同时,花朵开放过程中总香气物成分、法呢烯含量高峰提前;外施GA3使花朵开放过程中JsTPS2、JsTPS3、JsTPS4基因表达峰值提高,JsTPS1基因的表达水平降低;外施GA3后,在花朵开放过程中花香相关转录因子JsMYB305、JsMYB86、JsMYB108的表达水平提高,JsMYC2的表达水平降低,而基因JsbHLH13的表达水平提高。研究结果可为施加植物生长调节物质提高茉莉花香气提供理论依据。

关键词: 茉莉; 赤霉素; 萜类物质; TPS; MYB; bHLH

本文引用格式

ZHAO Wei-shu , WAN Chao , HU Li , ZHANG Qu , YUAN Yuan . 外施GA3对茉莉花主要萜类物质及其相关基因表达的影响[J]. 亚热带植物科学, 2022 , 51(4) : 241 -247 . DOI: 10.3969/j.issn.1009-7791.2022.04.001

Abstract

In this study, 100 μmol·L–1 gibberellin (GA3) was used to spray jasmine, and its effects on the main aroma components of jasmine and the expression levels of aroma synthesis-related structural genes and transcription factors were analyzed. The results showed that external GA3 could increase the total aroma components and the contents of linalool and farnesene during the blooming process, and make the peaks of total aroma components and farnesene content earlier during the blooming process. GA3 treatment increased the expression peaks of JsTPS2, JsTPS3, and JsTPS4 genes during flower opening, while the expression level of JsTPS1 decreased. After external application of GA3, the expression levels of floral aroma-related transcription factors JsMYB305, JsMYB86, and JsMYB108 increased during flower opening. The expression level of JsMYC2 decreased during flower opening, while the expression level of JsbHLH13 increased. The research results could provide a theoretical reference for improving the aroma of jasmine through plant growth regulators.

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