烟草根系烟碱代谢关键酶活性与基因表达对复合肥用量及其基肥比例的响应

ZHOU Jun-cheng; GUO Ya-li; CHEN Fa-yuan; ZHANG Heng; ZHU Di; PENG San-xi; GAO Huan-ye

doi:10.3969/j.issn.1009-7791.2022.05.001

亚热带植物科学 >

2022 , Vol. 51 >Issue 5: 331 - 339

DOI: https://doi.org/10.3969/j.issn.1009-7791.2022.05.001

植物生理生化与分子生物学

烟草根系烟碱代谢关键酶活性与基因表达对复合肥用量及其基肥比例的响应

ZHOU Jun-cheng ,
GUO Ya-li ,
CHEN Fa-yuan ,
ZHANG Heng ,
ZHU Di ,
PENG San-xi ,
GAO Huan-ye

展开

(1. 贵州大学烟草学院 / 贵州省烟草品质研究重点实验室, 贵州贵阳 550025；2. 贵州省烟草公司黔西南州公司，贵州兴义 562400)

收稿日期: 2022-08-27

录用日期: 2022-09-19

网络出版日期: 2023-01-17

基金资助

贵州省烟草公司黔西南州公司科技计划项目(201902)

收起

Responses of Nicotine Key Enzyme Activities and Gene Expression to Compound Fertilizer Dosage and its Proportion in Basal Fertilizer

周俊成，郭亚利，陈发元，张恒，朱迪，彭三喜，高焕晔

Expand

(1. College of Tobacco, Guizhou University / Key Laboratory of Tobacco Quality Research of Guizhou Province, Guiyang 550025, Guizhou China; 2. Guizhou Tobacco Company Qianxinanzhou Company, Xingyi 562400, Guizhou China)

Received date: 2022-08-27

Accepted date: 2022-09-19

Online published: 2023-01-17

Fold

摘要

以‘云烟87’(Nicotiana tabacum ‘Yunyan 87’)为材料，采用田间小区试验，设置3个烟草专用复合肥用量(6.25 kg·667 m–2、8.25 kg·667 m–2和10.25 kg·667 m–2)和3个基肥比例(50%、60%和70%)，探究烟草专用复合肥用量及基肥比例对其根系烟碱代谢关键酶ODC、ADC、MPO、PMT、QPRT活性及叶片基因NtODC、NtADC表达量的影响。结果表明，在烟草各生育期，不同用肥量及基肥比例对烟碱关键酶活性及基因表达的影响有所差异；旺长期前，不同复合肥用量及基肥比例对ODC、ADC、PMT、QPRT活性的影响均未达显著水平，而MPO活性随复合肥用量的增加总体呈下降趋势，随基肥比例的增大呈单峰变化。现蕾后，复合肥用量高的植株ODC和MPO活性表现较好，其中较适宜的基肥比例分别为50%、60%；ADC活性则在常规复合肥用量下较好，且对于同一复合肥用量下，又以50%基肥的活性较高；而PMT活性在常规和高复合肥用量下，以70%基肥处理较好；QPRT则表现出较大差异，QPRT活性在常规复合肥下随基肥比例的增加而降低，而高复合肥用量下则随基肥比例的增加而增强。旺长期、现蕾期的烟碱基因NtADC和NtODC表达量大致以高复合肥用量、低基肥比例处理的较高，常规施肥次之。

关键词： 烟草专用复合肥; 基肥比例; 烟碱; 酶活性; 基因表达量

本文引用格式

ZHOU Jun-cheng , GUO Ya-li , CHEN Fa-yuan , ZHANG Heng , ZHU Di , PENG San-xi , GAO Huan-ye . 烟草根系烟碱代谢关键酶活性与基因表达对复合肥用量及其基肥比例的响应[J]. 亚热带植物科学, 2022 , 51(5) : 331 -339 . DOI: 10.3969/j.issn.1009-7791.2022.05.001

Abstract

In order to explore the effect of tobacco-specific compound fertilizer dosage and base fertilizer ratio on the activities of nicotine metabolism key enzymes in roots, i.e. ODC, ADC, MPO, PMT and QPRT, and the expression of genes NtODC and NtADC in leaves of Nicotiana tabacum plants were carried out. In this experiment, a field test was adopted, using ‘Yunyan 87’ as the material, and 3 levels of tobacco-specific compound fertilizer dosage (6.25 kg·667 m–2, 8.25 kg·667 m–2and 10.25 kg·667 m–2) and 3 basal fertilizer ratios (50%, 60% and 70%). The results showed that the effects of different compound fertilizer amounts and basal fertilizer ratios on nicotine key enzyme activities and gene expression were different in each growth period; before the peak period, different compound fertilizer amounts and basal fertilizer ratios had different effects on the activities of ODC, ADC, PMT and QPRT. The significance analysis did not reach a significant level, while the activity of MPO before the peak period generally showed a downward trend with the increase of the compound fertilizer amount, and showed a single peak change with the increase of the base fertilizer ratio. After sprouting, the activities of ODC and MPO performed better at high compound fertilizer rates, and the more suitable basal fertilizer ratios were 50% and 60%, respectively; ADC activity was better with conventional compound fertilizer dosage, and for the same compound fertilizer rate, the ADC activity was higher when the base fertilizer ratio was 50% in general; while the PMT activity was better when the base fertilizer ratio was 70% under the conventional and high compound fertilizer dosages; The activity of QPRT decreased with the increase of base fertilizer proportion under conventional compound fertilizer, but increased with the increase of base fertilizer proportion under high compound fertilizer dosage. The expression levels of nicotine genes NtADC and NtODC in the prosperous and budding periods were generally higher under high compound fertilizer dosage and low basal fertilizer ratio, followed by conventional fertilization.

Key words： tobacco special compound fertilizer; base fertilizer ratio; nicotine; enzyme activity; gene expression

参考文献

[1] 黄树永, 陈良存. 烟草碳氮代谢研究进展[J]. 河南农业科学, 2005(4): 8–11.

[2] 鲁黎明, 曾孝敏, 顾会战, 张启莉, 喻晓, 王栋, 何佶弦, 李立芹. 施氮量对烤烟氮代谢关键酶基因表达及代谢产物的影响[J]. 浙江农业学报, 2018, 30(3): 454–460.

[3] 杨永霞, 石冰瑾, 王霄龙, 冯琦, 张松涛, 崔红. 不同海拔高度的烤烟淀粉合成动态研究[J]. 湖南农业大学学报(自然科学版), 2012, 38(1): 22–26.

[4] Ju X T, Chao F C. LC J, Jiang R F, P Christire, Zhang F S. Yield and nicotine content of fue-cured tobacco as affected by soil nitrogen miniralaition[J]. Pedosphere, 2008, 18(2): 227–235

[5] Rawson H M, Hackett C. An exploration of the carbon e-conomy of the tobacco plant ш. Gas exchange of leaves inrelation to position on the stem, ontogeny and nitrogencontent[J]. Australian Journal of Physiology, 1974(1): 552–560.

[6] 任昭辉,于大鹏, 安金花, 杭秋瑜, 王慧颖. 不同施氮量对晒红烟生理特性、干物质积累及化学成分的影响[J]. 延边大学农学学报, 2014, 36(1): 50–55.

[7] 韩锦峰. 烟草栽培生理[M]. 北京: 中国农业出版社, 2003: 179–182.

[8] 胡国松, 李志勇, 穆琳, 韩锦峰. 烤烟烟碱累积特点研究[J]. 中国烟草学报, 2000(2): 7–10.

[9] 王广山, 尹启生, 张树模, 谭辞平, 张祥林. 运用栽培技术措施调节白肋烟氮碱比提高其可用性的研究[J]. 烟草科技, 2001(6): 38–42.

[10] 谢志坚, 涂书新, 张嵚, 李海蓝, 韩丹, Muhammad I, 徐昌旭, 刘光荣, 罗华汉. 影响烤烟烟碱合成与代谢的因素及其机理分析[J].核农学报, 2014, 28(4): 714–719.

[11] Ray F D. Accumulation of nicotine in reciprocal grafts of tomato and tobacco[J]. American Journal of Botany, 1942, 29(1): 66–71.

[12] 王鹏, 罗朝鹏, 盖江涛. 植物鸟氨酸脱羧酶家族基因鉴定及进化分析[J]. 分子植物育种, 2018, 16(1): 96–101.

[13] 王威威, 席飞虎, 杨少峰, 江丽芳, 王峰吉. 烟草烟碱合成代谢调控研究进展[J]. 亚热带农业研究, 2016, 12(1): 62–67.

[14] 尹鹏嘉, 周世奇, 任民, 孙鑫, 罗成刚, 杨爱国, 张玉, 陈爱国, 李晓旭, 文柳璎. 烤烟苗期烟碱含量与其代谢基因的相关性分析[J].分子植物育种, 2018, 16(20): 6753–6760.

[15] 王鹏, 罗朝鹏, 盖江涛. 茄科植物甲基腐胺氧化酶基因进化路径分析[J]. 分子植物育种, 2018, 16(1): 47–53.

[16] Shi Q M, Li C J, Zhang F S. Nicotine synthesis in Nicotiana tabacum L. induced by mechanical wounding is regulated by auxin[J]. Journal of Experimental Botany, 2006, 57(11): 2899–907.

[17] 刘彪, 罗万麟, 戚兴莉, 高恒. 烟草鸟氨酸脱羧酶的生物信息学分析[J]. 热带农业科学, 2020, 40(6): 59–63.

[18] 金云峰, 李军营, 张建波, 龚明. 烟草烟碱代谢的生化和分子机制及其调控[J]. 基因组学与应用生物学, 2015, 34(4): 882–891.

[19] Mizusaki S, Tanabe Y, Noguchi M. Phytochemical studies on tobacco alkaloids ⅩⅣ. The occurrence and properties of putrescine N-methyltransferase in tobacco roots[J]. Plant and Cell Physiology, 1971, 12(4): 633–640.

[20] Sato F, Hashimoto T, Hachiya A. Metabolic engineering of plant alkaloid biosynthesis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2001, 98(1): 367–372.

[21] 张敏, 顾勇, 赵锦超, 彭勇, 张永辉, 徐传涛, 谢强, 夏建华, 张军杰, 鲁黎明. 烟叶烟碱含量与腐胺N-甲基转移酶基因表达间的相关性[J]. 贵州农业科学, 2017, 45(9): 1–4.

[22] 吴宇瑶, 谢瑞, 杨友成, 刘坤华, 聂琼. 转录因子NtMYC2b参与烟草烟碱含量杂种优势形成的机制研究[J]. 河南农业科学, 2019, 48(11): 45–53.

[23] Feth F, Wagner R, Wagner K G. Regulation in tobacco callus of enzyme activities of the nicotine pathway[J]. Planta, 1986, 168(3): 402–407.

[24] 梁思威. 外源物质降低白肋烟TSNAs含量及其机制的研究[D]. 武汉: 华中农业大学硕士学位论文, 2013.

[25] 江峰. 调控技术对烤烟烟碱积累和分配的研究[D]. 长沙: 湖南农业大学硕士学位论文, 2007.

[26] 韩锦峰. 烟草栽培生理[M]. 北京: 中国农业出版社, 1996.

[27] 丁福章, 李继新, 袁有波, 雷波. 烟草不同组织总RNA的提取方法初探[J]. 中国农学通报, 2007(12): 98–101.

[28] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method[J]. Methods, 2001, 25(4): 402–408.

[29] 范江, 杨继洪, 王守旗, 肖志新, 梁梅, 李海平, 邱尧. 打顶对烤烟生长和各器官尼古丁积累分配的影响[J]. 作物研究, 2016,(1): 36–40.

[30] 卞士权. 烟草转录因子NtMYB305a调控尼古丁合成的分子机制研究[D]. 北京: 中国农业科学院硕士学位论文, 2021.

[31] 杜致辉. NtQPT基因对烟草形态和品质形成的影响研究[D]. 贵阳: 贵州大学硕士学位论文, 2018.

[32] Wagner R, Feth F, Wagner K G. Regulation in tobacco callus of enzyme activities of the nicotine pathway[J]. Planta, 1986, 168: 408–413.

[33] Feth F, Wagner R, Wagner K G. Regulation in tobacco callus of enzyme activities of the nicotine pathway[J]. Planta, 1986, 168: 402–407.

[34] 金云峰, 李军营, 张建波, 龚明. 烟草尼古丁代谢的生化和分子机制及其调控[J]. 基因组学与应用生物学, 2015(4): 882–891.

[35] 黄化刚, 申燕, 吴飞跃, 杨振智, 刘世碧, 喻奇伟, 贾宏昉. 不同氮素来源对烤烟上部叶碳氮代谢及其基因表达的影响[J]. 南方农业学报, 2018, 49(3): 462–468.

Options

文章导航

〈

〉

闽ICP备11008787号-5　

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献