To study the effects of drought stress on physiological and biochemical characteristics and the differential expression of NtDEGP5 gene in different tobacco cultivars, drought resistant variety NC82 and drought sensitive Yunyan 87 were used as materials, and PEG-6000 was used to treat seedlings with drought stress. Fv/Fm, SOD activity, POD activity, MDA content and NtDEGP5 relative expression of two tobacco cultivars were determined after treatment, so as to provide a basis for further research on the function of NtDEGP5 gene. The results showed that drought stress had effects on Fv/Fm, SOD activity, POD activity and MDA content of tobacco seedlings. As the drought continued, Fv/Fm showed a gradual decline trend, while POD, SOD activity and MDA content showed a trend of first increasing and then decreasing. The response of different varieties to drought stress was different. Under drought stress for 0 to 9 hours, the Fv/Fm of Yunyan 87 seedlings decreased more than that of NC82, while the POD, SOD activity and MDA content increased less than that of NC82. The difference was not significant after 12 hours of drought stress. Under drought stress, the expression of NtDEGP5 was different among varieties and organs. The expression levels in roots, stems and leaves of Yunyan 87 seedlings were very low, and that in roots of NC82 seedlings was also very low, but it was high in leaves and stems after treatment for 9–12 h. NtDEGP5 expression in leaves of NC82 was positively correlated with Fv/Fm, POD activity and MDA content. The results showed that Fv/Fm, SOD activity, POD activity and MDA content could be used as drought resistance evaluation indicators of tobacco seedlings. NtDEGP5 gene expression was significantly correlated with drought resistance evaluation indicators. It was speculated that NtDEGP5 had drought resistance function.
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