基于全基因组的穿心莲NRAMP基因家族鉴定与生物信息学分析

FAN Pan-hui; WU Li-wei; SUN Wei; WANG Yu; GAO Wei; XING Jian-yong; SONG Jing-yuan; YAO Hui

doi:10.3969/j.issn.1009-7791.2023.03.001

亚热带植物科学 >

2023 , Vol. 52 >Issue 3: 175 - 182

DOI: https://doi.org/10.3969/j.issn.1009-7791.2023.03.001

植物生理生化与分子生物学

基于全基因组的穿心莲NRAMP基因家族鉴定与生物信息学分析

FAN Pan-hui ,
WU Li-wei ,
SUN Wei ,
WANG Yu ,
GAO Wei ,
XING Jian-yong ,
SONG Jing-yuan ,
YAO Hui

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(1. 中国医学科学院北京协和医学院药用植物研究所国家中医药管理局中药资源保护重点研究室，北京 100193；2. 中国中医科学院中药研究所，北京 100700；3. 华润三九(六安)中药材产业发展有限公司，安徽六安 237000；4. 华润三九现代中药制药有限公司，广东惠州 516000；5. 中药资源教育部工程研究中心，北京 100193)

收稿日期: 2023-04-24

录用日期: 2023-05-15

网络出版日期: 2023-08-24

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Identification and Bioinformation Analysis of NRAMP Gene Family Based on Andrographis paniculata Genome

范潘慧，武立伟，孙伟，王瑀，高巍，邢建永，宋经元，姚辉

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(1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China; 2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 3. China Resources Sanjiu (Lu’an) Traditional Chinese Medicine Industry Development Co., Ltd., Lu’an 237000, Anhui China; 4. China Resources Sanjiu Modern Chinese Medicine Pharmaceutical Co., Ltd., Huizhou 516000, Guangdong China; 5. Engineering Research Center of Chinese, Beijing 100193, China)

Received date: 2023-04-24

Accepted date: 2023-05-15

Online published: 2023-08-24

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摘要

自然抗性相关巨噬细胞蛋白(Natural resistance associated macrophage protein，NRAMP)是一类广泛存在于动植物及微生物中重要的膜转运蛋白，在植物重金属离子转运和重新利用中起着重要作用。为探究穿心莲Andrographis paniculata的NRAMP基因家族生物学特性，本研究根据拟南芥NRAMP家族的蛋白序列，从穿心莲全基因组中筛选鉴定了7个NRAMP家族成员，命名为ApNRAMP1～ApNRAMP7，并分析其基因结构和编码蛋白的理化性质等相关信息。结果表明，ApNRAMPs基因编码蛋白的长度为513～553 aa，分子量在56.00～60.54 kD之间，所有成员均定位于细胞膜上。系统进化分析表明，NRAMP基因家族分为2个亚家族。基因结构分析发现，属于GroupⅡ的ApNRAMP3～ApNRAMP6含有3个内含子，而属于GroupⅠ的ApNRAMP1、ApNRAMP2、ApNRAMP7则含有12个内含子；保守基序分析发现，Motif1～8保守基序存在于所有的ApNRAMPs编码的蛋白序列。蛋白结构预测分析发现，ApNRAMPs蛋白二级结构主要由α-螺旋和无规则卷曲构成，三维结构较为相似。该研究结果为进一步解析ApNRAMPs基因参与镉胁迫响应的分子机制提供参考。

本文引用格式

FAN Pan-hui , WU Li-wei , SUN Wei , WANG Yu , GAO Wei , XING Jian-yong , SONG Jing-yuan , YAO Hui . 基于全基因组的穿心莲NRAMP基因家族鉴定与生物信息学分析[J]. 亚热带植物科学, 2023 , 52(3) : 175 -182 . DOI: 10.3969/j.issn.1009-7791.2023.03.001

Abstract

Natural resistance associated macrophage protein (NRAMP) is a major class of membrane transporters widely found in animals, plants and microorganisms, and play an important role in transporting and reusing heavy metal ions in plants. For exploring the biological characteristics of NRAMP gene family in Andrographis paniculata, seven NRAMP gene family members were screened and identified from the whole genome, named ApNRAMP1–ApNRAMP7, and their gene structures and physicochemical properties of the encoded proteins were analyzed. The results showed that the proteins encoded by ApNRAMPs genes ranged from 513 aa to 553 aa in length, and their molecular weight were between 56.00 kD and 60.54 kD. The subcellular localizations of all members were in the cell membrane. Phylogenetic analysis found that the NRAMP gene family was divided into two subfamilies. ApNRAMP3–ApNRAMP6 in Group Ⅰ contained 3 introns, while ApNRAMP1, ApNRAMP2, and ApNRAMP7 in Group Ⅱ contained 12 introns, and their encoded protein sequences all contained Motif1–8 sequences. Protein structure prediction analysis showed that the secondary structures of ApNRAMPs proteins were mainly composed of α-helix and random coil, and the three-dimensional structures were relatively similar. This study provides a reference for further elucidation of the molecular mechanism of ApNRAMPs genes involvement in cadmium stress response.

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