植物生理生化与分子生物学

基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱分析簕菜化学成分

  • CHEN Lan-qing ,
  • LI Shu-zhen ,
  • GAN Li-she ,
  • JIN Jing-wei ,
  • WU Ri-hui ,
  • MA Hang ,
  • ZHANG Kun ,
  • LI Dong-li
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  • (五邑大学生物科技与大健康学院,广东 江门 529000)

收稿日期: 2023-04-17

  录用日期: 2023-06-25

  网络出版日期: 2023-11-28

基金资助

广东普通高校重点领域(乡村振兴战略)项目(2019KZDZX2003)

Identification of Chemical Components of Acanthopanax trifoliatus by UPLC-Q-Orbitrap HRMS

  • 陈兰青,黎淑贞,甘礼社,金静维,吴日辉,马 航,张 焜,李冬利
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  • (School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529000, Guangdong China)

Received date: 2023-04-17

  Accepted date: 2023-06-25

  Online published: 2023-11-28

摘要

建立分析簕菜Acanthopanax trifoliatus中多种化学成分的超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)法,为簕菜的质量评价提供科学依据。采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱联用仪(UPLC-Q-Orbitrap HRMS),选用Hypersil GOLD C18色谱柱(100 mm×2.1 mm,1.9 μm)进行分离,以0.1%甲酸水溶液-0.1%甲酸乙腈溶液体系进行梯度洗脱,质谱设置成负离子全扫描模式和二级质谱自动触发扫描,快速识别并通过数据库、文献对比以及对照品确认鉴定簕菜样品中的化学成分。共鉴定出29个化合物,其中19个多酚类化合物和10个萜类化合物。该研究首次报道使用UPLC-Q-Orbitrap HRMS方法快速全面分析簕菜化学成分的可行性,为簕菜活性化学成分分析提供参考。

本文引用格式

CHEN Lan-qing , LI Shu-zhen , GAN Li-she , JIN Jing-wei , WU Ri-hui , MA Hang , ZHANG Kun , LI Dong-li . 基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱分析簕菜化学成分[J]. 亚热带植物科学, 2023 , 52(4) : 301 -309 . DOI: 10.3969/j.issn.1009-7791.2023.04.004

Abstract

An ultra-high performance liquid chromatography-quadrupole/electrostatic field-orbital-trap-high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) method for the analysis of various chemical components in Acanthopanax trifoliatus was established. The sample was separated by Hypersil GOLD C18 column (100 mm × 2.1 mm, 1.9 μm), eluting with 0.1% formic acid acetonitrile-0.1% formic acid aqueous solution. Chemical components were quickly identified by mass spectrometry and comfirmed by the negative ion full scan mode and the secondary mass spectrometer automatic trigger scan. The chemical components were identified by database, literature comparison and reference materials. A total of 29 chemical components were identified, including nineteen polyphenolic compounds and ten terpenoids. This study was the first to report the feasibility of using the UPLC-Q-Orbitrap HRMS method to quickly and comprehensively analyze the active chemical components of A. trifoliatus. The method can provide a basis for the analysis of active chemical composition of A. trifoliatus.

参考文献

[1] 张元, 王真史, 何燕, 杜志云, 张焜. 响应面优化法提取白簕总多酚及抗氧化研究[J]. 食品工业, 2015, 36(11): 118–122.
[2] 张焜, 程书朋, 武文敬, 胡浩鹏, 林惠琼, 陈喜财, 李冬利, 何燕. 簕菜化学成分、生物活性及其安全性研究进展[J]. 海峡药学, 2017, 29(4): 1–5.
[3] 江森, 何燕, 李冬利, 张元, 王真史, 张焜, 杜志云. 一种簕菜凉茶的研制及抗氧化活性研究[J]. 食品研究与开发, 2013, 34(20): 33–37.
[4] 黄晓慧, 黄俊生, 衷明华, 刘岱纯, 郑德和. 白簕含氟牙膏的制作[J]. 韩山师范学院学报, 2007, 89(3): 74–76, 81.
[5] 刘岱纯, 黄俊生, 衷明华, 黄晓慧, 郑德和. 白簕透明香皂的制备工艺[J]. 江西化工, 2007, 86(2): 80–81.
[6] 黄俊生, 郑德和, 黄晓慧, 刘岱纯. 白簕消炎喷雾剂的研制[J]. 安徽农业科学, 2008, 36(32): 14155–14156.
[7] 张焜, 杜志云, 李晨悦, 胡瑞连, 王辉, 卢宇靖, 王华倩, 郑俊霞, 黄华容, 方岩雄, 赵肃清, 郑希, 汪舰, 曾华强, 艾伦·康尼. 一种含簕菜乙醇提取物的肤用化妆品[P]. 中国: CN102961275B, 2014–07–16.
[8] 陈小菊. 簕菜疗法在新生儿湿疹治疗中的应用和护理成效[J]. 实用医学杂志, 2006, 22(13): 1587–1589.
[9] Sithisarn P, Muensaen S, Jarikasem S. Determination of caffeoyl quinic acids and flavonoids in Acanthopanax trifoliatus leaves by HPLC [J]. Natural Product Communications, 2011, 6: 1289–1291.
[10] Chen Z F, Cheng S P, Lin H Q, Wu W J, Liang L Y, Chen X C, Zheng X, He Y, Zhang K. Antibacterial, anti-inflammatory, analgesic, and hemostatic activities of Acanthopanax trifoliatus (L.) Merr. [J]. Food Science & Nutrition, 2021, 9(4): 2191–2202.
[11] 吴丹, 黄碧云, 王静, 刘龙. 超高效液相色谱–四级杆/静电场轨道阱高分辨质谱法同时检测补中益气丸中毛蕊异黄酮葡萄糖苷、橙皮苷、甘草酸铵的含量[J]. 山东科学, 2023, 36(2): 16–22.
[12] 余文怡, 伍慧敏, 郭秀洁, 闫淑梅, 刘湘杰, 王竹君, 王超然, 沈爱金, 梁鑫淼. 基于超高效液相色谱–四极杆–静电场轨道阱高分辨质谱的刺五加注射液化学成分分析[J]. 色谱, 2023, 41(3): 207–223.
[13] Yang X J, Yang L, Xiong A Z, Li D X, Wang, Z T. Authentication of Senecio scandens and S. vulgaris based on the comprehensive secondary metabolic patterns gained by UPLC-DAD/ESI-MS [J]. Journal of Pharmaceutical and Biomedical Analysis, 2011, 56(2): 165–172.
[14] Matsui Y, Nakamura S, Kondou N, Takasu Y, Ochia R, Masukawa Y. Liquid chromatography-electrospray ionization- tandem mass spectrometry for simultaneous analysis of chlorogenic acids and their metabolites in human plasma [J]. Journal of Chromatography B- Analytical Technologies in the Biomedical and Life Sciences, 2007, 858(1–2): 96–105.
[15] Zhang W J, Xu M, Yu C Q, Zhang G F, Tang X. Simultaneous determination of vitexin-4 ''-O-glucoside, vitexin-2 ''-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC-ESI-MS/MS [J]. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2010, 878(21): 1837–1844.
[16] 第五文博, 谈娜娜, 王晓玲, 王乐. 元宝枫叶中的黄酮类成分分析与电喷雾质谱裂解规律研究[J]. 宝鸡文理学院学报(自然科学版), 2020, 40(3): 25–33.
[17] Li D L, Zheng X, Chen Y C, Jiang S, Zhang Y, Zhang W M, Wang H Q, Du Z Y, Zhang K. Terpenoid composition and the anticancer activity of Acanthopanax trifoliatus [J]. Archives of Pharmacal Research, 2016, 39(1): 51–58.
[18] 王宝庆, 郭鑫磊, 刘莹, 练有扬, 张乔. 白簕化学成分及其药理活性研究进展[J]. 北方园艺, 2018(13): 162–168.
[19] Chen M, Qin Y, Ma H, Zheng X, Zhou R P, Sun S L, Huang Y Q, Duan Q, Liu W F, Wu P P, Xu X T, Sheng Z J, Zhang K, Li D L. Downregulating NF-κB signaling pathway with triterpenoids for attenuating inflammation: in vitro and in vivo studies [J]. Food & function, 2019, 10(8): 5080–5090.
[20] Li D L, Du Z Y, Li C Y, Liu Y, Goodin S, Huang H R, He Y, Zhang Y, Wang H Q, Zheng X. Potent inhibitory effect of terpenoids from Acanthopanax trifoliatus on growth of PC-3 prostate cancer cells in vitro and in vivo is associated with suppression of NF-κB and STAT3 signalling [J]. Journal of Functional Foods, 2015, 15: 274–283.
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