Cutting is one of the important methods to propagate and preserve good varieties and resources, especially for self-incompatibility and cross-pollination alfalfa. Currently, there are some limitations in the application of current alfalfa cutting methods, such as complicated operation and long cycle. Based on the theory of root regeneration controlled by mitochondria, a simple and efficient new cutting method of alfalfa, named semi-wet cutting method was proposed in this study. This method can be completed only by covering the cuttings with moisturizing material and placing them in a container. In this experiment, two alfalfa varieties, ‘Zhongmu No. 1’ and ‘Xinjiang Daye’, were selected and cultured by semi-wet method and hydroponics method, aiming at comparing the rooting effect of different varieties under semi-wet method and the difference between the two cutting methods. The experimental results showed that there was no significant difference in the rooting rate of the two varieties by using semi-wet cutting, and the rooting rate was very high, more than 90% on the 8th day and close to 100% on the 10th day in both varieties, which was significantly faster than that by using hydroponic cutting. To sum up, semi-wet cutting not only simplified the operation process, but also effectively
shortened the rooting time, which has important practical application value for the propagation of alfalfa and the collection of field resources.
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